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Products:Signal Transduction >> Adapters >> Cytoplasmic
Overview
Product name
Anti-PACSIN3 antibody
See all PACSIN3 products (4) ...
Description
Rabbit polyclonal to PACSIN3
Tested applications
WB, IHC-P, ELISA, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
KLH-conjugated synthetic peptide selected from the sequence of human PACSIN3 within aa 1-100.
Positive control
Mouse brain tissue lysate; Human breast carcinoma tissue
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab37612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/100 - 1/500.Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/1000ELISA: 1/1000
ICC/IF: Use at an assay dependent dilution.
Target
Function
May play a role in vesicle formation and transport.
Tissue specificity
Expressed in heart and skeletal muscle, lung, kidney, adrenal gland and placenta.
Sequence similarities
Belongs to the PACSIN family.
Contains 1 FCH domain.
Contains 1 SH3 domain.
Post-translational
modifications
Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC).
Cellular localization
Cytoplasm. Cell membrane. Detected at the inner aspect of the plasma membrane in cells or myotubes.
Target information above from: UniProt accessionQ9UKS6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Endophilin 1 antibody
- Endophilin I antibody
- FLJ17861 antibody
see all
Database links
- Entrez Gene: 29763 Human
- Entrez Gene: 80708 Mouse
- Omim: 606513 Human
- SwissProt: Q9UKS6 Human
- SwissProt: Q99JB8 Mouse
- Unigene: 334639 Human
Anti-PACSIN3 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PACSIN3 antibody (ab37612)
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot - PACSIN3 antibody (ab37612)
Anti-PACSIN3 antibody (ab37612) at 1/100 dilution + Mouse brain tissue lysate.
Predicted band size : 48 kDa
Observed band size : 48 kDa
References for Anti-PACSIN3 antibody (ab37612)
ab37612 has not yet been referenced specifically in any publications.
Publishing research using ab37612? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"