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Western blot - PACSIN3 antibody (ab86077)
All lanes : Anti-PACSIN3 antibody (ab86077) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size : 47 kDa
Detection: chemiluminescence with exposure time of 30 seconds
Immunoprecipitation - PACSIN3 antibody (ab86077)
1mg whole cell lysate from HeLa cells was immunoprecipitated using 3µg ab86077. 20% of IP was loaded per lane, and probed with ab86077 at 1µg/ml (lane 1) or with a control IgG (lane 2). Detection: chemiluminescence with an exposure time of 3 seconds.
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