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Anti-PACSIN3 antibody (ab86077)

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Overview

Product name

Anti-PACSIN3 antibody
See all PACSIN3 products (4) ...

Description

Rabbit polyclonal to PACSIN3

Tested applications

WB, IPmore details

Cross reactivity

Reacts with

Human

Immunogen

Synthetic peptide, corresponding to a region between residues 325 and 375 of Human PACSIN3 (NP_057307.2)

Positive control

HeLa whole cell lysate.

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Western blot - PACSIN3 antibody (ab86077)Western blot - PACSIN3 antibody (ab86077) image (enlarge)

  • Immunoprecipitation - PACSIN3 antibody (ab86077)Immunoprecipitation - PACSIN3 antibody (ab86077) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab86077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

May play a role in vesicle formation and transport.

Tissue specificity

Expressed in heart and skeletal muscle, lung, kidney, adrenal gland and placenta.

Sequence similarities

Belongs to the PACSIN family.
Contains 1 FCH domain.
Contains 1 SH3 domain.

Post-translational
modifications

Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC).

Cellular localization

Cytoplasm. Cell membrane. Detected at the inner aspect of the plasma membrane in cells or myotubes.

Target information above from: UniProt accessionQ9UKS6 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Endophilin 1 antibody
  • Endophilin I antibody
  • FLJ17861 antibody
  • OTTHUMP00000234262 antibody
  • OTTHUMP00000234263 antibody
  • OTTHUMP00000234264 antibody
  • PACN3_HUMAN antibody
  • Pacsin3 antibody
  • Protein kinase C and casein kinase substrate in neurons 3 antibody
  • Protein kinase C and casein kinase substrate in neurons protein 3 antibody
  • SDPIII antibody
  • SH3 domain containing protein 6511 antibody
  • SH3 domain-containing protein 6511 antibody
  • syndapin III antibody
see all

Anti-PACSIN3 antibody images:

  Western blot - PACSIN3 antibody (ab86077)

Western blot - PACSIN3 antibody (ab86077)

All lanes : Anti-PACSIN3 antibody (ab86077) at 0.04 µg/ml

Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg


Predicted band size : 47 kDa


Detection: chemiluminescence with exposure time of 30 seconds

  Immunoprecipitation - PACSIN3 antibody (ab86077)

Immunoprecipitation - PACSIN3 antibody (ab86077)

1mg whole cell lysate from HeLa cells was immunoprecipitated using 3µg ab86077.
20% of IP was loaded per lane, and probed with ab86077 at 1µg/ml (lane 1) or with a control IgG (lane 2).
Detection: chemiluminescence with an exposure time of 3 seconds.

References for Anti-PACSIN3 antibody (ab86077)

ab86077 has not yet been referenced specifically in any publications.

Publishing research using ab86077? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"