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Western blot - PACSIN3 antibody (ab86077)
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Immunoprecipitation - PACSIN3 antibody (ab86077)
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Product Name
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PACSIN3 antibody
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See all PACSIN3 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to PACSIN3
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Immunogen
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Synthetic peptide, corresponding to a region between residues 325 and 375 of Human PACSIN3 (NP_057307.2)
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: Use at 2 - 5µg/mg of lysate.
WB: 1/2000 - 1/10000. Predicted molecular weight: 47 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa whole cell lysate.
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Cellular localization
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Cytoplasmic. Detected at the inner aspect of the plasma membrane in cells or myotubes.
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Research areas
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Signal Transduction >> Adapters >> Cytoplasmic
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Relevance
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PACSIN3 differs from the other family members in having a short proline-rich region and lacking asparagine-proline-phenylalanine motifs. In contrast to the neurospecific PACSIN1 and the ubiquitously expressed PACSIN2, PACSIN3 is mainly detected in lung and muscle tissues. PACSIN3 may play a role in vesicle formation and transport.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PACSIN3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PACSIN3 antibody images, references, products related to ab86077 and other tools.
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PACSIN3 antibody images:
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 Western blot - PACSIN3 antibody (ab86077)
All lanes : PACSIN3 antibody (ab86077) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size : 47 kDa
Detection: chemiluminescence with exposure time of 30 seconds
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Immunoprecipitation - PACSIN3 antibody (ab86077)
1mg whole cell lysate from HeLa cells was immunoprecipitated using 3µg ab86077.
20% of IP was loaded per lane, and probed with ab86077 at 1µg/ml (lane 1) or with a control IgG (lane 2).
Detection: chemiluminescence with an exposure time of 3 seconds.
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Search PubMed (MEDLINE) for references to PACSIN3
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PACSIN3 antibody - more information
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Customer reviews (feedback) regarding PACSIN3 antibody |
Customer FAQs regarding PACSIN3 antibody |
Protocols for PACSIN3 antibody |
Price and availability of products related to PACSIN3 antibody |
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PACSIN3 antibody - related products:
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PACSIN3 antibody - other tools:
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Contact Abcam with a Technical Enquiry about PACSIN3 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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