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Products:Signal Transduction >> Adapters >> Cytoplasmic
Overview
Product name
Anti-PACSIN3 antibody
See all PACSIN3 products (4) ...
Description
Rabbit polyclonal to PACSIN3
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide, corresponding to a region between residues 325 and 375 of Human PACSIN3 (NP_057307.2)
Positive control
HeLa whole cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab86077 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000.Predicted molecular weight: 47 kDa.
IP: Use at 2-5 µg/mg of lysate.
Target
Function
May play a role in vesicle formation and transport.
Tissue specificity
Expressed in heart and skeletal muscle, lung, kidney, adrenal gland and placenta.
Sequence similarities
Belongs to the PACSIN family.
Contains 1 FCH domain.
Contains 1 SH3 domain.
Post-translational
modifications
Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC).
Cellular localization
Cytoplasm. Cell membrane. Detected at the inner aspect of the plasma membrane in cells or myotubes.
Target information above from: UniProt accessionQ9UKS6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Endophilin 1 antibody
- Endophilin I antibody
- FLJ17861 antibody
see all
Database links
- Entrez Gene: 29763 Human
- Omim: 606513 Human
- SwissProt: Q9UKS6 Human
- Unigene: 334639 Human
Anti-PACSIN3 antibody images:
Western blot - PACSIN3 antibody (ab86077)
All lanes : Anti-PACSIN3 antibody (ab86077) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size : 47 kDa
Detection: chemiluminescence with exposure time of 30 seconds
Immunoprecipitation - PACSIN3 antibody (ab86077)
1mg whole cell lysate from HeLa cells was immunoprecipitated using 3µg ab86077. 20% of IP was loaded per lane, and probed with ab86077 at 1µg/ml (lane 1) or with a control IgG (lane 2). Detection: chemiluminescence with an exposure time of 3 seconds.
References for Anti-PACSIN3 antibody (ab86077)
ab86077 has not yet been referenced specifically in any publications.
Publishing research using ab86077? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"