Anti-PADI2 / PAD2 antibody (ab16478)

Western blot - PADI2 / PAD2 antibody (ab16478)

All lanes : Anti-PADI2 / PAD2 antibody (ab16478) at 1 µg/ml

Lane 1 : Human Kidney Lysate
Lane 2 : Human Kidney Lysate with PADI2 / PAD2 peptide (ab17091) at 1 µg/ml

Lysates/proteins at 20 µg per lane.


Predicted band size : 45 kDa
Observed band size : 43 kDa (why is the actual band size different from the predicted?)


ab16478 recognises a specific band at 43kDa corresponding to PADI2, which is specifically blocked using the immunizing peptide (ab17091).

The length of PADI2 is currently uncertain. According to Strausberg et al. (2002) [PMID: 12477932] it is 437aa in length (~45kDa). However, according to Ishigami et al. (2002) [PMID: 12392711] PADI2 is 665aa (~75kDa).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI2 / PAD2 antibody (ab16478)

Image courtesy of Human Protein Atlas

ab16478 staining PADI2 in female rectum, showing a distinct and strong staining pattern in glandular cells. Paraffin embedded human rectal tissue was incubated with ab16478 (1/100 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

ab16478 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

Immunocytochemistry/ Immunofluorescence - PADI2 / PAD2 antibody (ab16478)

ICC/IF image of ab16478 stained human Hek293 cells. The cells were PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab16478, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

Flow Cytometry - PADI2 / PAD2 antibody (ab16478)

ab16478 staining human peripheral blood mononuclear cells (cultured with M-CSF) by Flow Cytometery. Cells were treated with flow cytometery staining buffer (PBS 0.1% sodium azide 1% BSA) and gating was done on myeloid cells. The primary antibody was diluted 1/10 (PBS 0.1% sodium azide 1% BSA) and incubated with sample for 20 minutes at 25°C. An Alexa Fluor® conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.

This image is courtesy of an Abreview submitted by Dr Frances Santiago-Schwarz