|
|
|
|
Western blot - PADI2 / PAD2 antibody (ab56075)
(enlarge)
|
|
|
|
|
Product Name
|
PADI2 / PAD2 antibody
|
| |
See all PADI2 / PAD2 antibodies (4)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to PADI2 / PAD2
|
|
Immunogen
|
Synthetic peptide corresponding to C terminal residues of human PADI2 / PAD2 (peptidyl arginine deiminase, type II)
|
|
Reacts with
(species key)
|
Hu, Ms
|
|
Tested applications
(see key)
|
ELISA, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 µg/ml for 2 hrs. Predicted molecular weight: 76 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Cellular localization
|
Cytoplasmic and Nuclear
|
|
Research areas
|
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Arginine Deimination
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Arginine Deiminases
Signal Transduction >> Metabolism >> Amino Acids
|
|
Relevance
|
PADI2 / PAD2 is a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. Each family member has a distinct substrate specificity and tissue expression pattern. The type II enzyme is the most widely expressed family member. Known substrates for this enzyme include myelin basic protein in the central nervous system and vimentin in skeletal muscle and macrophages. This enzyme is thought to play a role in the onset and progression of neurodegenerative disorders, including Alzheimers disease and multiple sclerosis, and it has also been implicated in the pathogenesis of glaucoma.
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.01% Sodium Azide
Constituents: 50% Glycerol, PBS Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
|
|
Purification notes
|
Purified by antigen specific affinity chromatography.
|
|
Form
|
Liquid
|
|
Concentration
|
0.500 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PADI2 / PAD2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for PADI2 / PAD2 antibody images, references, products related to ab56075 and other tools.
|
| |
PADI2 / PAD2 antibody images:
|
 Western blot - PADI2 / PAD2 antibody (ab56075)
All lanes : PADI2 / PAD2 antibody (ab56075) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 76 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)
The length of PADI2 is currently uncertain. According to Strausberg et al. (2002) [PMID: 12477932] it is 437aa in length (~45kDa). However, according to Ishigami et al. (2002) [PMID: 12392711] PADI2 is 665aa (~75kDa).
|
| |
| |
|
Search PubMed (MEDLINE) for references to PADI2 / PAD2
|
| |
PADI2 / PAD2 antibody - more information
|
Customer reviews (feedback) regarding PADI2 / PAD2 antibody |
Customer FAQs regarding PADI2 / PAD2 antibody |
Protocols for PADI2 / PAD2 antibody |
Price and availability of products related to PADI2 / PAD2 antibody |
| |
PADI2 / PAD2 antibody - related products:
|
|
|
| |
PADI2 / PAD2 antibody - other tools:
|
Contact Abcam with a Technical Enquiry about PADI2 / PAD2 antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
