Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Overview
Product name
PADI2 / PAD2 peptide
Protein description
Synthetic peptide derived from within residues 100 - 200 of Human PADI2 / PAD2. (Note: the amino acid sequence is proprietary) This peptide was used as an immunogen for ab16478 .
Protein length
437 amino acids
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Information available upon request.
Concentration
Concentration information loading...
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Arginine Deimination
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Arginine Deiminases
Signal Transduction >> Metabolism >> Amino Acids
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
-
Western blot - PADI2 / PAD2 peptide (ab17091)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab17091 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking
Application notes
BL: Use at a concentration of 1 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
Function
Catalyzes the deimination of arginine residues of proteins.
Sequence similarities
Belongs to the protein arginine deiminase family.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ9Y2J8
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
PADI2 / PAD2 peptide images:
Western blot - PADI2 / PAD2 peptide (ab17091)
All lanes : Anti-PADI2 / PAD2 antibody (ab16478) at 1 µg/ml
Lane 1 : Human Colon Lysate
Lane 2 : Human Skeletal Muscle Lysate
Lane 3 : Human Kidney Lysate
Lane 4 : Human Colon Lysate with PADI2 / PAD2 peptide (ab17091) at 1 µg/ml
Lane 5 : Human Skeletal Muscle Lysate with PADI2 / PAD2 peptide (ab17091) at 1 µg/ml
Lane 6 : Human Kidney Lysate with PADI2 / PAD2 peptide (ab17091) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
ab16478 recognises a specific band at 43kDa corresponding to PADI2, which is specifically blocked using the immunizing peptide (ab17091).
The length of PADI2 is currently uncertain. According to Strausberg et al. (2002) [PMID: 12477932] it is 437aa in length (~45kDa). However, according to Ishigami et al. (2002) [PMID: 12392711] PADI2 is 665aa (~75kDa).
There is a non-specific band recognised in human skeletal muscle lysate at 18kDa, which is attributed to a cross-reactivity.
References for PADI2 / PAD2 peptide (ab17091)
ab17091 has not yet been referenced specifically in any publications.
Publishing research using ab17091? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"