Anti-PADI4 / PAD4 antibody (ab96758)
Key features and details
- Rabbit polyclonal to PADI4 / PAD4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PADI4 / PAD4 antibody
See all PADI4 / PAD4 primary antibodies -
Description
Rabbit polyclonal to PADI4 / PAD4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment containing a sequence corresponding to a region within amino acids 326-581 of Human PADI4/ PAD4 (AAH25718).
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Positive control
- WB: Transfected HEK-293 T whole cell lysate, human spleen tissue extract; IHC-P: Cal27 xenograft and mouse ovary tissue; IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96758 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/10000. Predicted molecular weight: 74 kDa.
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IHC-P | (1) |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/10000. Predicted molecular weight: 74 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Catalyzes the citrullination/deimination of arginine residues of proteins. Citrullinates histone H3 at 'Arg-8' and/or 'Arg-17' and histone H4 at 'Arg-3', which prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1. -
Tissue specificity
Expressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes. -
Involvement in disease
Genetic variations in PADI4 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Note=Could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context. -
Sequence similarities
Belongs to the protein arginine deiminase family. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasmic granule. Cytoplasmic granules of eosinophils and neutrophils. - Information by UniProt
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Database links
- Entrez Gene: 23569 Human
- Entrez Gene: 18602 Mouse
- Omim: 605347 Human
- SwissProt: Q9UM07 Human
- SwissProt: Q9Z183 Mouse
- Unigene: 522969 Human
- Unigene: 250358 Mouse
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Alternative names
- EC 3.5.3.15 antibody
- HL 60 PAD antibody
- HL-60 PAD antibody
see all
Images
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All lanes : Anti-PADI4 / PAD4 antibody (ab96758) at 1/5000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : PAD4-transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 74 kDa7.5% SDS-PAGE
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Immunohistochemical analysis of paraffin-embedded Cal27 xenograft tissue staining PADI4/PAD4 with ab96758 at 1/100 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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Immunofluorescence analysis of paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) whole cells
labeling PADI4/PAD4 with ab96758 at 1/200 dilution. -
Anti-PADI4 / PAD4 antibody (ab96758) at 1/1000 dilution + Human spleen tissue extract at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 74 kDa7.5% SDS-PAGE
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Immunohistochemical analysis of paraffin-embedded mouse ovary tissue staining PADI4/PAD4 at cytoplasm with ab96758 at 1/500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab96758 has been referenced in 6 publications.
- Talal S et al. Neutrophil degranulation and severely impaired extracellular trap formation at the basis of susceptibility to infections of hemodialysis patients. BMC Med 20:364 (2022). PubMed: 36284314
- Ou Q et al. TcpC inhibits neutrophil extracellular trap formation by enhancing ubiquitination mediated degradation of peptidylarginine deiminase 4. Nat Commun 12:3481 (2021). PubMed: 34108482
- Ni Y et al. Interruption of neutrophil extracellular traps formation dictates host defense and tubular HOXA5 stability to augment efficacy of anti-Fn14 therapy against septic AKI. Theranostics 11:9431-9451 (2021). PubMed: 34646379
- Kosgodage US et al. Peptidylarginine Deiminase Inhibitors Reduce Bacterial Membrane Vesicle Release and Sensitize Bacteria to Antibiotic Treatment. Front Cell Infect Microbiol 9:227 (2019). PubMed: 31316918
- Liang Y et al. Inhibition of peptidylarginine deiminase alleviates LPS-induced pulmonary dysfunction and improves survival in a mouse model of lethal endotoxemia. Eur J Pharmacol 833:432-440 (2018). PubMed: 29981294
- Kolodziej S et al. PADI4 acts as a coactivator of Tal1 by counteracting repressive histone arginine methylation. Nat Commun 5:3995 (2014). ChIP, WB ; Human . PubMed: 24874575