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Products:Signal Transduction >> Metabolism >> Amino Acids
Overview
Product name
Anti-PADI4 / PAD4 antibody
See all PADI4 / PAD4 products (8) ...
Description
Goat polyclonal to PADI4 / PAD4
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: AQGTLIRVTPEQP-C, corresponding to N terminal amino acids 2-14 of Human PADI4 / PAD4
AQGTLIRVTP EQP-C
Positive control
Human spleen and human liver lysates for Western blot. Tansfected HEK293 transiently expressing PADI4 / PAD4.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab26071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.3 - 1 µg/ml.Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).Can be blocked with PADI4 / PAD4 peptide (2-14) (ab30475).
Target
Relevance
In vivo expression of PADI4 correlates with the appearance of citrulline in histones. It has been demonstrated that PADI4 converts arginines in the histone H3 tail to citrulline, blocks transcriptional activation and is recruited to the endogenous promotor. PADI4 represents a novel class of histone modifying enzymes probably linked to oestrogen regulated transcription. PADI4 is thought to be a rheumatoid arthritis susceptibility locus, and could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context.
Cellular localization
Cytoplasm. Nucleus. Cytoplasmic granule. Note: Cytoplasmic granules of eosinophils and neutrophils.
Alternative names
- EC 3.5.3.15 antibody
- HL 60 PAD antibody
- PAD 4 antibody
see all
Database links
- Entrez Gene: 23569 Human
- GenBank: NP_036519 Human
- Omim: 605347 Human
- SwissProt: Q5VTZ7 Human
- SwissProt: Q5VTZ8 Human
- SwissProt: Q9UM07 Human
- Unigene: 522969 Human
Anti-PADI4 / PAD4 antibody images:
Western blot - PADI4 / PAD4 antibody (ab26071)
Anti-PADI4 / PAD4 antibody (ab26071) at 0.3 µg/ml + human spleen lysate (35µg protein in RIPA buffer).
developed using the ECL technique
Predicted band size : 74 kDa
Observed band size : 74 kDa
Primary incubation was 1 hour.
Western blot - PADI4 / PAD4 antibody (ab26071)
Anti-PADI4 / PAD4 antibody (ab26071) at 1 µg/ml + transfected HEK293 transiently expressing PADI4 / PAD4
Predicted band size : 74 kDa
Observed band size : 74 kDa
References for Anti-PADI4 / PAD4 antibody (ab26071)
This product has been referenced in:
- Kinloch Aet al. Synovial fluid is a site of citrullination of autoantigens in inflammatory arthritis. Arthritis Rheum 58:2287-95 (2008). WB; Human.Read more (PubMed: 18668562) »
See 1 publication for this product
Publishing research using ab26071? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"