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Western blot - PADI4 / PAD4 antibody (ab26071)
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Product Name
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PADI4 / PAD4 antibody
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See all PADI4 / PAD4 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to PADI4 / PAD4
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Immunogen
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Synthetic peptide: AQGTLIRVTPEQP-C, corresponding to N terminal amino acids 2-14 of Human PADI4 / PAD4
BLAST 'AQGTLIRVTPEQP-C' with  or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/64000.
WB: Use at a concentration of 0.3 - 2 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human spleen and human liver lysates for Western blot.
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Cellular localization
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Cytoplasmic granules.
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Research areas
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Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Arginine Deimination
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Arginine Deiminases
Signal Transduction >> Metabolism >> Amino Acids
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Relevance
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In vivo expression of PADI4 correlates with the appearance of citrulline in histones. It has been demonstrated that PADI4 converts arginines in the histone H3 tail to citrulline, blocks transcriptional activation and is recruited to the endogenous promotor. PADI4 represents a novel class of histone modifying enzymes probably linked to oestrogen regulated transcription. PADI4 is thought to be a rheumatoid arthritis susceptibility locus, and could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide.
Constituents: 0.5% BSA, TBS. pH 7.3. Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PADI4 / PAD4 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PADI4 / PAD4 antibody images, references, products related to ab26071 and other tools.
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PADI4 / PAD4 antibody images:
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 Western blot - PADI4 / PAD4 antibody (ab26071)
PADI4 / PAD4 antibody (ab26071) at 0.3 µg/ml + Human Spleen lysate (35µg protein in RIPA buffer).
developed using the ECL technique
Predicted band size : 74 kDa
Observed band size : 74 kDa
Primary incubation was 1 hour.
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Search PubMed (MEDLINE) for references to PADI4 / PAD4
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PADI4 / PAD4 antibody - more information
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Customer reviews (feedback) regarding PADI4 / PAD4 antibody |
Customer FAQs regarding PADI4 / PAD4 antibody |
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PADI4 / PAD4 antibody - related products:
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PADI4 / PAD4 antibody - other tools:
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Contact Abcam with a Technical Enquiry about PADI4 / PAD4 antibody |
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