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Western blot - PADI4 / PAD4 antibody (ab38772)
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Immunocytochemistry/ Immunofluorescence - PADI4 / PAD4 antibody (ab38772)
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Product Name
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PADI4 / PAD4 antibody
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See all PADI4 / PAD4 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to PADI4 / PAD4
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human PADI4 / PAD4. (Peptide available as ab38771.)
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
WB: 1/250. Detects a band of approximately 67 kDa (predicted molecular weight: 74 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive result in the following whole cell lysates:
HeLa (Human epithelial carcinoma cell line)
Jurkat (Human T cell lymphoblast-like cell line)
A431 (Human epithelial carcinoma cell line)
HEK 293 (Human embryonic kidney cell line)
HepG2 (Human hepatocellular liver carcinoma cell line)
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Cellular localization
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Cytoplasmic granules.
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Research areas
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Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Arginine Deimination
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Arginine Deiminases
Signal Transduction >> Metabolism >> Amino Acids
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Relevance
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In vivo expression of PADI4 correlates with the appearance of citrulline in histones. It has been demonstrated that PADI4 converts arginines in the histone H3 tail to citrulline, blocks transcriptional activation and is recruited to the endogenous promotor. PADI4 represents a novel class of histone modifying enzymes probably linked to oestrogen regulated transcription. PADI4 is thought to be a rheumatoid arthritis susceptibility locus, and could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.10 - 0.10 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PADI4 / PAD4 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PADI4 / PAD4 antibody images, references, products related to ab38772 and other tools.
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PADI4 / PAD4 antibody images:
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 Immunocytochemistry/ Immunofluorescence - PADI4 / PAD4 antibody (ab38772)
ICC/IF image of ab38772 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab38772, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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Search PubMed (MEDLINE) for references to PADI4 / PAD4
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PADI4 / PAD4 antibody - more information
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Customer reviews (feedback) regarding PADI4 / PAD4 antibody |
Customer FAQs regarding PADI4 / PAD4 antibody |
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PADI4 / PAD4 antibody - related products:
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PADI4 / PAD4 antibody - other tools:
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