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Western blot - PADI4 / PAD4 antibody (ab38772)
Western blot - PADI4 / PAD4 antibody (ab38772)
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Immunocytochemistry/ Immunofluorescence - PADI4 / PAD4 antibody (ab38772)
Immunocytochemistry/ Immunofluorescence - PADI4 / PAD4 antibody (ab38772)
(enlarge)


Product Name 

PADI4 / PAD4 antibody

 

See all PADI4 / PAD4 antibodies (7)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to PADI4 / PAD4

Immunogen 

Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human PADI4 / PAD4.

(Peptide available as ab38771.)

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ICC/IF, WB


Abreviews 

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(see key)


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Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
WB: 1/250. Detects a band of approximately 67 kDa (predicted molecular weight: 74 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

This antibody gave a positive result in the following whole cell lysates:
HeLa (Human epithelial carcinoma cell line)
Jurkat (Human T cell lymphoblast-like cell line)
A431 (Human epithelial carcinoma cell line)
HEK 293 (Human embryonic kidney cell line)
HepG2 (Human hepatocellular liver carcinoma cell line)

Cellular localization 

Cytoplasmic granules.

Research areas 

Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Arginine Deimination
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Arginine Deiminases
Signal Transduction >> Metabolism >> Amino Acids

Relevance 

In vivo expression of PADI4 correlates with the appearance of citrulline in histones. It has been demonstrated that PADI4 converts arginines in the histone H3 tail to citrulline, blocks transcriptional activation and is recruited to the endogenous promotor. PADI4 represents a novel class of histone modifying enzymes probably linked to oestrogen regulated transcription. PADI4 is thought to be a rheumatoid arthritis susceptibility locus, and could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context.

 

Alternative names PADI4 / PAD4 antibody (ab38772)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

23569    (Human)

GenBank

    

NP_036519    (Human)

Omim

    

605347    (Human)

SwissProt

    

Q9UM07    (Human)
Q5VTZ7    (Human)
Q5VTZ8    (Human)

Unigene

    

522969    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Batch dependent within range: 0.10 - 0.10 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

Find concentration of your lot :

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PADI4 / PAD4 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for PADI4 / PAD4 antibody images, references, products related to ab38772 and other tools.

 

PADI4 / PAD4 antibody images:

Product image  Western blot - PADI4 / PAD4 antibody (ab38772)

Western blot - PADI4 / PAD4 antibody (ab38772)

All lanes : PADI4 / PAD4 antibody (ab38772) at 1/250 dilution

Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)
Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate (ab7902)
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate (ab7900)

Lysates/proteins at 10 µg per lane.

Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Performed under reducing conditions.

Predicted band size : 74 kDa
Observed band size : 67 kDa (why is the actual band size different from the predicted?)


Full length PADI4 has been shown to migrate at 67 kDa.  Please refer to the reference below for further details.

Chang X et al. Localization of peptidylarginine deiminase 4 (PADI4) and citrullinated protein in synovial tissue of rheumatoid arthritis. Rheumatology (Oxford) 44:40-50 (2005). PMID:15466895.

    

 

Product image  Immunocytochemistry/ Immunofluorescence - PADI4 / PAD4 antibody (ab38772)

Immunocytochemistry/ Immunofluorescence - PADI4 / PAD4 antibody (ab38772)

ICC/IF image of ab38772 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab38772, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

 
 

Search PubMed (MEDLINE) for references to PADI4 / PAD4

 

PADI4 / PAD4 antibody - more information

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PADI4 / PAD4 antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Immunizing Peptide for PADI4 / PAD4 antibody

ab38771

  

PADI4 / PAD4 peptide

 

Compatible Secondaries for PADI4 / PAD4 antibody

ab6564

  

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ab6717

  

FITC antibody

ab6720

  

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ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

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Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other PADI4 / PAD4 Antibodies

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PADI4 / PAD4 antibody - other tools:

Contact Abcam with a Technical Enquiry about PADI4 / PAD4 antibody

 

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