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Products:Signal Transduction >> Metabolism >> Amino Acids
Overview
Product name
Anti-PADI4 / PAD4 antibody
See all PADI4 / PAD4 products (8) ...
Description
Rabbit polyclonal to PADI4 / PAD4
Specificity
This antibody reacts specifically with the 74 kDa PADI4 / PAD4 protein. It shows no cross reactivity with the other isoforms.
Tested applications
WB, IP, ELISA, ICC/IFmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
A 15 amino acid synthetic peptide derived from the C-terminal domain of human PADI4 / PAD4.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
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Immunocytochemistry/ Immunofluorescence - Anti-PADI4 / PAD4 antibody (ab50247)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab50247 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/200 - 1/2000.Predicted molecular weight: 74 kDa.
IP
IP: 1/20 - 1/100.IP: 1/20 - 1/100.
ELISA
ELISA: 1/1000 - 1/5000.ELISA: 1/1000 - 1/5000.
ICC/IF: 1/200
Target
Relevance
In vivo expression of PADI4 correlates with the appearance of citrulline in histones. It has been demonstrated that PADI4 converts arginines in the histone H3 tail to citrulline, blocks transcriptional activation and is recruited to the endogenous promotor. PADI4 represents a novel class of histone modifying enzymes probably linked to oestrogen regulated transcription. PADI4 is thought to be a rheumatoid arthritis susceptibility locus, and could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context.
Cellular localization
Cytoplasm. Nucleus. Cytoplasmic granule. Note: Cytoplasmic granules of eosinophils and neutrophils.
Alternative names
- EC 3.5.3.15 antibody
- HL 60 PAD antibody
- PAD 4 antibody
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Database links
- Entrez Gene: 23569 Human
- Entrez Gene: 18602 Mouse
- Entrez Gene: 29512 Rat
- GenBank: NP_036519 Human
- Omim: 605347 Human
- SwissProt: Q5VTZ7 Human
- SwissProt: Q5VTZ8 Human
- SwissProt: Q9UM07 Human
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Anti-PADI4 / PAD4 antibody images:
Immunocytochemistry/ Immunofluorescence - Anti-PADI4 / PAD4 antibody (ab50247)
ICC/IF image of ab50247 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab50247, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-PADI4 / PAD4 antibody (ab50247)
ab50247 has not yet been referenced specifically in any publications.
Publishing research using ab50247? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"