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Anti-PADI4 / PAD4 antibody (ab50332)

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1 question for ab50332

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Question 1

Wednesday 20-August-2008

I want to duplicate the "HL60 cells induced with 1.25% DMSO for three days", experiment as shown in your WB image, Lane 4. Can you please forward the experimental protocols?

ANSWER:

  

Thank you for your enquiry.

The exact protocols are as below:

1. DMSO was added to a concentration of 1.25% to normal HL60 media. This would be the equivalent to adding 125ul DMSO to 10ml media. No additional buffers were required.

2. The HL60 cells were normally at a 50% confluence at the point when the DMSO was added.

3. Cells were incubated, as normal, for another 3 days after the addition of 1.25% DMSO.

I hope this information helps. If there is anything else that I can help you with, please do not hesitate to contact me.

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