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Products:Signal Transduction >> Metabolism >> Amino Acids
Overview
Product name
Anti-PADI4 / PAD4 antibody
See all PADI4 / PAD4 products (8) ...
Description
Rabbit polyclonal to PADI4 / PAD4
Tested applications
IP, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to amino acids 1-15 of human PADI4, conjugated to KLH via a C-terminal added cysteine residue
MAQGTLIRVT PEQPT
Positive control
Extracts of DMSO-treated HL60 cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Ion Exchange Chromatography
Clonality
Polyclonal
Isotype
IgG
-
Western blot - PADI4 / PAD4 antibody (ab50332)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab50332 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution. (2-4 µl of the antibody immunoprecipitatesPADI4 from DMSO-treated HL60 cells)
WB: 1/1000 - 1/2000.Predicted molecular weight: 74 kDa.
Target
Relevance
In vivo expression of PADI4 correlates with the appearance of citrulline in histones. It has been demonstrated that PADI4 converts arginines in the histone H3 tail to citrulline, blocks transcriptional activation and is recruited to the endogenous promotor. PADI4 represents a novel class of histone modifying enzymes probably linked to oestrogen regulated transcription. PADI4 is thought to be a rheumatoid arthritis susceptibility locus, and could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context.
Cellular localization
Cytoplasm. Nucleus. Cytoplasmic granule. Note: Cytoplasmic granules of eosinophils and neutrophils.
Alternative names
- EC 3.5.3.15 antibody
- HL 60 PAD antibody
- PAD 4 antibody
see all
Database links
- Entrez Gene: 23569 Human
- GenBank: NP_036519 Human
- Omim: 605347 Human
- SwissProt: Q5VTZ7 Human
- SwissProt: Q5VTZ8 Human
- SwissProt: Q9UM07 Human
- Unigene: 522969 Human
Anti-PADI4 / PAD4 antibody images:
Western blot - PADI4 / PAD4 antibody (ab50332)
All lanes : Anti-PADI4 / PAD4 antibody (ab50332) at 1/1000 dilution
Lane 1 : 293-T cells
Lane 2 : 293-T cells transfected with PADI4 cDNA
Lane 3 : HL60 cells
Lane 4 : HL60 cells induced with 1.25% DMSO for three days
Secondary
Goat Anti-Rabbit IgG
Predicted band size : 74 kDa
Observed band size : ~62 kDa (why is the actual band size different from the predicted?)
References for Anti-PADI4 / PAD4 antibody (ab50332)
This product has been referenced in:
- Tanikawa Cet al. Regulation of protein Citrullination through p53/PADI4 network in DNA damage response. Cancer Res 69:8761-9 (2009). WB; Human.Read more (PubMed: 19843866) »
See 1 publication for this product
Publishing research using ab50332? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"