Anti-PADPR antibody [10H] (ab14459)

  Immunohistochemistry (Frozen sections) - PADPR antibody [10H] (ab14459)

Immunohistochemistry of rat livers treated with diethylnitrosamine (200 mg/kg) and stained with ab14459 diluted 1/100. After treatment livers were removed and rapidly processed 10 hr later, at peak polymer induction. Left hand side image was from diethylnitrosamine untreated liver tissue and right one represents DEN treated sections.

  Immunocytochemistry/ Immunofluorescence - PADPR antibody [10H] (ab14459)

ab14459 staining PADPR in human breast cancer cells (MCF7 cells) by Immunocytochemistry/ Immunofluorescence. The cells were paraformaldehyde fixed, permeabilised in 0.2% Triton/ PBS and then blocked using 1% BSA and 3% FBS in PBS for 30 minutes at room temperature. Samples were then incubated with primary antibody at 1/50 for 1 hour. The secondary antibody used was a goat anti-mouse IgG conjugated to Alexa Fluor® 555 (red) used at a 1/500 dilution.

Image courtesy of Dr Manikandan Periyasamy by Abreview.

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  Western blot - Anti-PADPR antibody [10H] (ab14459)

All lanes : Anti-PADPR antibody [10H] (ab14459) at 1/1000 dilution

Lane 1 : Ladder
Lane 2 : Ladder
Lane 3 : Whole cell lysate prepared from MEF's
Lane 4 : Whole cell lysate prepared from MEF's treated with 500um Hydrogen Peroxide, 10 minutes
Lane 5 : Whole cell lysate prepared from MEF's treated with 500um Hydrogen Peroxide, 20 minutes
Lane 6 : Whole cell lysate prepared from MEF's treated with 500um Hydrogen Peroxide, 30 minutes

Secondary
IRDye 800CW conjugated goat monoclonal at 1/10000 dilution

Image courtesy of Dr Aashish Joshi by Abreview.

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