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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab52720? Please let us know so that we can cite the reference in this datasheet
ab52720 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - PAF1 antibody (ab52720)
All lanes : Anti-PAF1 antibody (ab52720) at 1/1000 dilution
Lane 1 : total protein extract from E coli with ~50ng to 100 ng of a fusion protein of an irrelevant antigen.
Lane 2 : total protein extract from E coli with ~50ng to 500ng of the antigen (antigen fusion protein).
Lysates/proteins at 20 µg per lane.
Secondary
Rabbit anti-mouse IgG + IgM, (H+L) conjugated to Horseradish peroxidase at 1/5000 dilution
Predicted band size : 60 kDa
Note: the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.
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