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Product Name
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PAF1 antibody
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See all PAF1 antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to PAF1
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Immunogen
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Synthetic peptide corresponding to a region between residues 475 and 525 of Human PAF1 (TrEMBL entry Q9NUU9)
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms (Due to sequence homology)
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Tested applications
(see key)
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IP
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Abreviews
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Application notes
(see key)
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Recommended dilutions IP: Use at 3-10µg/mg of lysate.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Whole cell lysate from 293T cells
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Elongation factors Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes Chromatin and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> Polymerase
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Relevance
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In yeast, PAF1 acts as a docking protein and is involved in transcriptional elongation through interactions with RNA polymerase II and other members of the transcription machinery. The human homolog has also been shown to interact with RNA polymerase II, in particular with the form which is phosphorylated on Serine 2 or 5 in the carboxy-terminal domain (CTD). It also associates with HRPT2 (parafibromin) and other members of the PAF1 complex (LEO1 and CTR9). This complex is involved in transcriptional elongation and 3' end processing. There is evidence that overexpression of PAF1 is involved in cancer development.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1% Sodium Azide Constituents: PBS, 8mM Phosphate, 60mM Citrate, 150mM Tris, pH 7-8 Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PAF1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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References for PAF1 antibody (ab70638)
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This product has been used in: (two most recent references)
Lee JH & Skalnik DG Wdr82 is a C-terminal domain-binding protein that recruits the Setd1A Histone H3-Lys4 methyltransferase complex to transcription start sites of transcribed human genes. Mol Cell Biol 28:609-18 (2008). PubMed: 17998332
Rozenblatt-Rosen O et al. The parafibromin tumor suppressor protein is part of a human Paf1 complex. Mol Cell Biol 25:612-20 (2005). PubMed: 15632063
If you publish research using ab70638 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to PAF1
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PAF1 antibody - more information
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