PAF1 antibody (ab70638)

Product Name 

PAF1 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to PAF1

Immunogen 

Synthetic peptide corresponding to a region between residues 475 and 525 of Human PAF1 (TrEMBL entry Q9NUU9)

Reacts with 

Hu

Predicted to react 
(species key)

Ms
(Due to sequence homology)

Tested applications 
(see key)

IP

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IP: Use at 3-10µg/mg of lysate.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Whole cell lysate from 293T cells

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Transcription >> Elongation factors
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Chromatin and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> Polymerase

Relevance 

In yeast, PAF1 acts as a docking protein and is involved in transcriptional elongation through interactions with RNA polymerase II and other members of the transcription machinery. The human homolog has also been shown to interact with RNA polymerase II, in particular with the form which is phosphorylated on Serine 2 or 5 in the carboxy-terminal domain (CTD). It also associates with HRPT2 (parafibromin) and other members of the PAF1 complex (LEO1 and CTR9). This complex is involved in transcriptional elongation and 3' end processing. There is evidence that overexpression of PAF1 is involved in cancer development.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: PBS, 8mM Phosphate, 60mM Citrate, 150mM Tris, pH 7-8

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C.

References for PAF1 antibody (ab70638)

  This product has been used in: (two most recent references)

Lee JH & Skalnik DG Wdr82 is a C-terminal domain-binding protein that recruits the Setd1A Histone H3-Lys4 methyltransferase complex to transcription start sites of transcribed human genes. Mol Cell Biol 28:609-18 (2008). PubMed: 17998332

Rozenblatt-Rosen O et al. The parafibromin tumor suppressor protein is part of a human Paf1 complex. Mol Cell Biol 25:612-20 (2005). PubMed: 15632063
If you publish research using ab70638 please let us know so that we can cite the reference on this datasheet.

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