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Products:Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> Polymerase
Overview
Product name
Anti-PAF1 antibody
See all PAF1 products (4) ...
Description
Rabbit polyclonal to PAF1
Tested applications
IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to a region between residues 475 and 525 of human PAF1 (TrEMBL Q9NUU9).
Positive control
Human controls: Anaplastic Thyroid Carcinoma, Bladder Cell Carcinoma, Breast Carcinoma, Colon Carcinoma, Laryngeal Squamous Cell Carcinoma, Non-Small Cell Lung Cancer, Ovarian Carcinoma, Pancreatic Islet Cell Tumor, Skin Melanoma and Small Cell Lung Cancer.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab84914 was affinity purified using an epitope specific to Paf1 immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Elongation factors
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> Polymerase
Applications
Show applications key
Our Abpromise guarantee covers the use of ab84914 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 2 Images
IHC-P
Application notes
IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
In yeast, PAF1 acts as a docking protein and is involved in transcriptional elongation through interactions with RNA polymerase II and other members of the transcription machinery. The human homolog has also been shown to interact with RNA polymerase II, in particular with the form which is phosphorylated on Serine 2 or 5 in the carboxy-terminal domain (CTD). It also associates with HRPT2 (parafibromin) and other members of the PAF1 complex (LEO1 and CTR9). This complex is involved in transcriptional elongation and 3' end processing. There is evidence that overexpression of PAF1 is involved in cancer development.
Cellular localization
Nuclear
Alternative names
- F23149 1 antibody
- FLJ11123 antibody
- PAF 1 antibody
see all
Database links
- Entrez Gene: 54623 Human
- Omim: 610506 Human
- SwissProt: Q9H166 Human
- Unigene: 466714 Human
Anti-PAF1 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAF1 antibody (ab84914)
ab84914, at a 1/250 dilution, staining PAF1 in formalin fixed, paraffin embedded human small cell lung cancer by Immunohistochemistry using DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAF1 antibody (ab84914)
ab84914, at 1/250 dilution, staining PAF1 in formalin fixed, paraffin embedded human pancreatic islet cell tumor by Immunohistochemistry using DAB staining.
References for Anti-PAF1 antibody (ab84914)
ab84914 has not yet been referenced specifically in any publications.
Publishing research using ab84914? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"