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Products:Immunology >> Innate Immunity >> Macrophage / Inflamm.
Overview
Product name
PAF2 protein (Active)
See all PAFAH products (2) ...
Protein description
Recombinant full length Human PAF2 produced in 293 cells; amino acids 22-441, 48kDa. Swiss ID = Q13093.
Properties
Purity
>95% by SDS-PAGE
Purification notes
Purity is greater than 95% by SDS-PAGE gel and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
Biological activity
Biological Activity : Measured by its ability to cleave a PAF analog in a chromogenic substrate linked assay. At a PAF2 concentration of 10.0 µg/ml, 50% cleavage was achieved at an incubation time of approximately 2 minutes.
Form
Lyophilised
Storage instructions
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Concentration
Concentration information loading...
Sequence notes
FDWQYINPVA HMKSSAWVNK IQVLMAAASF GQTKIPRGNG PYSVGCTDLM FDHTNKGTFL RLYYPSQDND RLDTLWIPNK EYFWGLSKFL GTHWLMGNIL RLLFGSMTTP ANWNSPLRPG EKYPLVVFSH GLGAFRTLYS AIGIDLASHG FIVAAVEHRD RSASATYYFK DQSAAEIGDK SWLYLRTLKQ EEETHIRNEQ VRQRAKECSQ ALSLILDIDH GKPVKNALDL KFDMEQLKDS IDREKIAVIG HSFGGATVIQ TLSEDQRFRC GIALDAWMFP LGDEVYSRIP QPLFFINSEY FQYPANIIKM KKCYSPDKER KMITIRGSVH QNFADFTFAT GKIIGHMLKL KGDIDSNVAI DLSNKASLAF LQKHLGLHKD FDQWDCLIEG DDENLIPGTN INTTNQHIML QNSSGIEKYN
Research areas
Immunology >> Secreted Molecules >> Other secreted molecules
Cardiovascular >> Blood >> Other
Cardiovascular >> Blood >> Platelets
Immunology >> Innate Immunity >> Macrophage / Inflamm.
Applications
Show applications key
Our Abpromise guarantee covers the use of ab82130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Functional Studies
SDS-PAGE
Application notes
FuncS: Use at an assay dependent dilution.
SDS-PAGE: Use at an assay dependent dilution.
Recombinant PAF2 is a 420 amino acid glycoprotein which migrates with an apparent molecular mass of 47-55 kDa by SDS-PAGE analysis.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
Relevance
Platelet activating factor (PAF) acetylhydrolase is a secreted enzyme that catalyzes the degradation of PAF to inactive products by hydrolysis of the acetyl group at the sn-2 position, producing the biologically inactive products LYSO-PAF and acetate.
Cellular localization
Secreted protein; extracellular space.
References for PAF2 protein (Active) (ab82130)
ab82130 has not yet been referenced specifically in any publications.
Publishing research using ab82130? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"