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Products:Cardiovascular >> Blood >> Platelets
Overview
Product name
Anti-PAFAH1B2 antibody
See all PAFAH1B2 products (2) ...
Description
Chicken polyclonal to PAFAH1B2
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Cow, Human
Immunogen
Synthetic peptide, corresponding to amino acids 1-229 of PAFAH1B2.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgY
Research areas
Signal Transduction >> Metabolism >> Lipid metabolism
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Hydrolysis
Cardiovascular >> Blood >> Platelets
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Western blot - PAFAH1B2 antibody (ab15875)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab15875 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF
- 1 Image
WB
Application notes
ICC/IF: Use at an assay dependent dilution (PMID 19395675).
WB: 1/500. Predicted molecular weight: 30 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Platelet-activating factor (PAF) is a biologically active phospholipid with diverse biologic effects. PAF is degraded to inactive products by hydrolysis of the acetyl group at the sn2 position to produce the biologically inactive products LYSO PAF and acetate. This reaction is catalyzed by PAF acetylhydrolase (PAFAH). The various monomeric and multimeric forms of the enzyme are composed of alpha, beta, and gamma PAFAH subunits. The catalytic activity of the enzyme resides in the beta and gamma subunits, whereas the alpha subunit has regulatory activity. Trimer formation is not essential for the catalytic activity.
Cellular localization
Cytoplasmic
Alternative names
- PAF acetylhydrolase 30 kDa subunit antibody
- PAF AH 30 kDa subunit antibody
- PAF AH beta subunit antibody
see all
Database links
- Entrez Gene: 282514 Cow
- Entrez Gene: 5049 Human
- Entrez Gene: 18475 Mouse
- Entrez Gene: 64189 Rat
- Omim: 602508 Human
- SwissProt: P68401 Cow
- SwissProt: P68402 Human
- SwissProt: Q6IBR6 Human
see all
Anti-PAFAH1B2 antibody images:
Western blot - PAFAH1B2 antibody (ab15875)
Predicted band size : 30 kDa
E coli derived fusion protein as test antigen. Ab15875 dilution: 1:2000. Colorimetric method for signal development
References for Anti-PAFAH1B2 antibody (ab15875)
This product has been referenced in:
- Bechler MEet al. The phospholipase complex PAFAH Ib regulates the functional organization of the Golgi complex. J Cell Biol 190:45-53 (2010). WB; Rat.Read more (PubMed: 20624900) »
- Ho Jet al. Novel breast cancer metastasis-associated proteins. J Proteome Res 8:583-94 (2009). WB; Human.Read more (PubMed: 19086899) »
See all 5 publications for this product
Publishing research using ab15875? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"