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Overview

  • Product nameAnti-PAFAH1B3 antibodySee all PAFAH1B3 primary antibodies ...
  • Description
    Mouse polyclonal to PAFAH1B3
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human PAFAH1B3 (NP_002564.1)

  • Positive controlHuman kidney tissue lysate, transfected 293T cells

Properties

Applications

Our Abpromise guarantee covers the use of ab88396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa.

Target

  • FunctionInactivates paf by removing the acetyl group at the sn-2 position. This is a catalytic subunit. Plays an important role during the development of brain.
  • Tissue specificityIn the adult, expressed in brain, skeletal muscle, kidney, thymus, spleen, colon, testis, ovary and peripheral blood leukocytes. In the fetus, highest expression occurs in brain.
  • Sequence similaritiesBelongs to the 'GDSL' lipolytic enzyme family. Platelet-activating factor acetylhydrolase IB beta/gamma subunits subfamily.
  • Cellular localizationCytoplasm.

    • Target information above from: UniProt accession Q15102 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        PA1B3_HUMAN antibodyPAF acetylhydrolase 29 kDa subunit antibodyPAF AH 29 kDa subunit antibody
        PAF AH subunit gamma antibodyPAF-AH 29 kDa subunit antibodyPAF-AH subunit gamma antibodyPAFAH subunit gamma antibodyPafah1b3 antibodyPAFAHG antibodyPlatelet activating factor acetylhydrolase IB subunit gamma antibodyPlatelet activating factor acetylhydrolase isoform Ib gamma subunit (29kD) antibodyPlatelet activating factor acetylhydrolase isoform Ib gamma subunit 29kDa antibodyPlatelet activating factor acetylhydrolase isoform Ib gamma subunit antibodyPlatelet-activating factor acetylhydrolase IB subunit gamma antibody
      see all

    Anti-PAFAH1B3 antibody images

    • Western blot - PAFAH1B3 antibody (ab88396)
      Western blot - PAFAH1B3 antibody (ab88396)
      All lanes : Anti-PAFAH1B3 antibody (ab88396) at 1 µg/ml

      Lane 1 : transfected 293T cell lysate
      Lane 2 : non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG at 1/5000 dilution

      Predicted band size : 26 kDa
      Observed band size : 26 kDa
    • Western blot - PAFAH1B3 antibody (ab88396)
      Western blot - PAFAH1B3 antibody (ab88396)
      Anti-PAFAH1B3 antibody (ab88396) at 1 µg/ml + human kidney tissue lysate at 50 µg

      Secondary
      Goat Anti-Mouse IgG at 1/5000 dilution

      Predicted band size : 26 kDa

    References for Anti-PAFAH1B3 antibody (ab88396)

    ab88396 has not yet been referenced specifically in any publications.

    Publishing research using ab88396 ? Please let us know so that we can cite the reference in this datasheet

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    Flow Cytometry abreview for Anti-PAFAH1B3 antibody

     Below Average
    Abreviews
    Application Flow Cytometry
    Sample Human Cell (Platelets)
    Specification Platelets
    Preparation Cell harvesting/tissue preparation method: PL were isolated spinning Platelet rich plasma on Histopaque
    Sample buffer: PBS
    Fixation Paraformaldehyde
    Permeabilization Yes - 0.1% Triton-X100 in 2% BSA for 30min
    Gating Strategy Platelets
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    Submitted Dec 17 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"