Overview

  • Product nameAnti-PAFAH1B3 antibodySee all PAFAH1B3 primary antibodies ...
  • Description
    Mouse polyclonal to PAFAH1B3
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human PAFAH1B3 (NP_002564.1)

  • Positive control
    • Human kidney tissue lysate, transfected 293T cells

Properties

Applications

Our Abpromise guarantee covers the use of ab88396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa.

Target

  • FunctionInactivates paf by removing the acetyl group at the sn-2 position. This is a catalytic subunit. Plays an important role during the development of brain.
  • Tissue specificityIn the adult, expressed in brain, skeletal muscle, kidney, thymus, spleen, colon, testis, ovary and peripheral blood leukocytes. In the fetus, highest expression occurs in brain.
  • Sequence similaritiesBelongs to the 'GDSL' lipolytic enzyme family. Platelet-activating factor acetylhydrolase IB beta/gamma subunits subfamily.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • PA1B3_HUMAN antibody
    • PAF acetylhydrolase 29 kDa subunit antibody
    • PAF AH 29 kDa subunit antibody
    • PAF AH subunit gamma antibody
    • PAF-AH 29 kDa subunit antibody
    • PAF-AH subunit gamma antibody
    • PAFAH subunit gamma antibody
    • Pafah1b3 antibody
    • PAFAHG antibody
    • Platelet activating factor acetylhydrolase IB subunit gamma antibody
    • Platelet activating factor acetylhydrolase isoform Ib gamma subunit (29kD) antibody
    • Platelet activating factor acetylhydrolase isoform Ib gamma subunit 29kDa antibody
    • Platelet activating factor acetylhydrolase isoform Ib gamma subunit antibody
    • Platelet-activating factor acetylhydrolase IB subunit gamma antibody
    see all

Anti-PAFAH1B3 antibody images

  • All lanes : Anti-PAFAH1B3 antibody (ab88396) at 1 µg/ml

    Lane 1 : transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG at 1/5000 dilution

    Predicted band size : 26 kDa
    Observed band size : 26 kDa
  • Anti-PAFAH1B3 antibody (ab88396) at 1 µg/ml + human kidney tissue lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG at 1/5000 dilution

    Predicted band size : 26 kDa

References for Anti-PAFAH1B3 antibody (ab88396)

ab88396 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Platelets)
Specification Platelets
Preparation Cell harvesting/tissue preparation method: PL were isolated spinning Platelet rich plasma on Histopaque
Sample buffer: PBS
Fixation Paraformaldehyde
Permeabilization Yes - 0.1% Triton-X100 in 2% BSA for 30min
Gating Strategy Platelets
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted Dec 17 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"