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Product Name
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PAG antibody [FOM-1]
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See all PAG antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rat monoclonal [FOM-1] to PAG
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Immunogen
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Full length native protein (purified) (Mouse)
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Reacts with
(species key)
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Ms
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Specificity
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Negative on monocytes and granulocytes and positive on macrophages in the red pulp of the spleen and the medulla of inguinal and axial lymph nodes. Reticular cells in the T-cell area of the spleen and lymph nodes are also positive. A subpopulation of positive macrophages appear in the peritoneal cavity in response to inflammatory stimuli. Other macrophages in lymphoid and non-lymphoid organs are negative.
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Tested applications
(see key)
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IHC-Fr
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-Fr: 1/100 - 1/400.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cell Membrane; Single-pass type III membrane protein. Note=Present in lipid rafts.
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Research areas
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Signal Transduction >> Adapters >> Transmembrane
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
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Relevance
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Csk binding protein, also known as PAG (phosphoprotein associated with GEMs), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.
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PAG antibody [FOM-1] (ab54133) |
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 94212 (Mouse)
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Raised in
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Rat
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Clonality
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Monoclonal
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Clone number
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FOM-1
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Isotype
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IgM
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 10mg/ml BSA, pH 7.2 Material safety datasheet (MSDS) for this product:
Thimerosal MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PAG antibody [FOM-1] is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to PAG
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PAG antibody [FOM-1] - more information
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