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Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Overview
Product name
Anti-PAG antibody [FOM-1]
See all PAG products (5) ...
Description
Rat monoclonal [FOM-1] to PAG
Specificity
Negative on monocytes and granulocytes and positive on macrophages in the red pulp of the spleen and the medulla of inguinal and axial lymph nodes. Reticular cells in the T-cell area of the spleen and lymph nodes are also positive. A subpopulation of positive macrophages appear in the peritoneal cavity in response to inflammatory stimuli. Other macrophages in lymphoid and non-lymphoid organs are negative.
Tested applications
IHC-Frmore details
Cross reactivity
Reacts with
Mouse
Immunogen
Full length native protein (purified) (Mouse)
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 10mg/ml BSA, pH 7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Monoclonal
Clone number
FOM-1
Isotype
IgM
Research areas
Signal Transduction >> Adapters >> Transmembrane
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab54133 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr
IHC-Fr: 1/100 - 1/400.IHC-Fr: 1/100 - 1/400.
Target
Function
Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling.
Tissue specificity
Ubiquitously expressed. Present in germinal center B-cells, plasma cells, T-cells, monocytes and platelets (at protein level).
Post-translational
modifications
Palmitoylated.
Phosphorylated by FYN on Tyr-317 in resting T-cells; which promotes interaction with CSK. Dephosphorylated by PTPRC/CD45 upon TCR activation; which leads to CSK dissociation. May also be dephosphorylated by PTPN11. Hyperphosphorylated in mast cells upon FCER1 activation.
Cellular localization
Cell membrane. Present in lipid rafts.
Target information above from: UniProt accessionQ9NWQ8
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CBP antibody
- Csk binding protein antibody
- Csk-binding protein antibody
see all
Database links
- Entrez Gene: 94212 Mouse
- SwissProt: Q3U1F9 Mouse
- Unigene: 23897 Mouse
References for Anti-PAG antibody [FOM-1] (ab54133)
ab54133 has not yet been referenced specifically in any publications.
Publishing research using ab54133? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"