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I am interested in your antibody against Csk binding protein - ab9527. I would like to know what antibody concentration was used and what protein load was used for western blotting protocols with this antibody. Thanks. |
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ANSWER: |
Thank you for your enquiry. CSK Binding Protein antibody Ref:ab9527 [MEM-255] should stain blots strongly in dilutions 1:500 - 1:1000 but optimum dilution should be done for a particular system of a researcher. We did not measure protein load, it always depends on the type of lysate and level of expression of the specific protein. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
1. Frozen Raji cells lysate, immunoprecipitated with ab4206
2. Fresh Raji cells lysate, immunoprecipitated with ab4206
3. Frozen Raji cells lysate, immunoprecipitated with ab14989
Reprobe with ab9527.
The immunoprecipitation used covalently bound antibody to Sepharose beads as immunosorbent. This method of precipitation obviously differs from proteinA/G-sepharose or GoatxMs -Sepharose mediated immunoprecipitation protocols.
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