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Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Overview
Product name
Anti-PAG antibody [MEM-255]
See all PAG products (5) ...
Description
Mouse monoclonal [MEM-255] to PAG
Specificity
This antibody is specific for CSK Binding Protein
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Recombinant intracellular fragment, corresponding to amino acids 97-432 of Human CSK Binding Protein.
Positive control
RAMOS and Jurkat cells
General notes
Csk-Binding Protein is also known as PAG (Protein Associated with Glycosphingolipid-enriched microdomains).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Purification notes
Purified from ascites by protein A-affinity chromatography.
Clonality
Monoclonal
Clone number
MEM-255
Isotype
IgG2a
Research areas
Signal Transduction >> Adapters >> Transmembrane
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
-
Immunoprecipitation
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: Use at an assay dependant dilution. Use a lysis buffer made with Tris HCl (pH 8.1) and 1%laurylmaltoside (with common protease inhibitors). Use under non-reducing conditions.
Csk binding protein is an ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane microdomains (rafts), which however migrates on SDS-PAGE gels anomalously as an 80 kDa molecule. The reason is, that PAG has a pI value of about 4.6 and binds poorely SDS and therefore it had decreased mobility on SDS gels.
Is unsuitable for IP (use ab4206 or ab14989).
May be unsuitable for ICC-IF due to unsuccesful results in submitted Abreview. (Please see Abreview comments)
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling.
Tissue specificity
Ubiquitously expressed. Present in germinal center B-cells, plasma cells, T-cells, monocytes and platelets (at protein level).
Post-translational
modifications
Palmitoylated.
Phosphorylated by FYN on Tyr-317 in resting T-cells; which promotes interaction with CSK. Dephosphorylated by PTPRC/CD45 upon TCR activation; which leads to CSK dissociation. May also be dephosphorylated by PTPN11. Hyperphosphorylated in mast cells upon FCER1 activation.
Cellular localization
Cell membrane. Present in lipid rafts.
Target information above from: UniProt accessionQ9NWQ8
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CBP antibody
- Csk Binding Protein antibody
- Csk-binding protein antibody
see all
Database links
- Entrez Gene: 55824 Human
- Omim: 605767 Human
- SwissProt: Q9NWQ8 Human
- Unigene: 266175 Human
Anti-PAG antibody [MEM-255] images:
Immunoprecipitation
1. Frozen Raji cells lysate, immunoprecipitated with ab4206
2. Fresh Raji cells lysate, immunoprecipitated with ab4206
3. Frozen Raji cells lysate, immunoprecipitated with ab14989
Reprobe with ab9527.
The immunoprecipitation used covalently bound antibody to Sepharose beads as immunosorbent. This method of precipitation obviously differs from proteinA/G-sepharose or GoatxMs -Sepharose mediated immunoprecipitation protocols.
References for Anti-PAG antibody [MEM-255] (ab9527)
This product has been referenced in:
- Mutch CMet al. Activation-induced endocytosis of the raft-associated transmembrane adaptor protein LAB/NTAL in B lymphocytes: evidence for a role in internalization of the B cell receptor. Int Immunol 19:19-30 (2007).Read more (PubMed: 17090619) »
- Brdickova Net al. Interaction between two adapter proteins, PAG and EBP50: a possible link between membrane rafts and actin cytoskeleton. FEBS Lett 507:133-6 (2001).Read more (PubMed: 11684085) »
See all 3 publications for this product
Publishing research using ab9527? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"