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Product Name
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PAG antibody [MEM-255]
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See all PAG antibodies (5)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [MEM-255] to PAG
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Immunogen
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Recombinant intracellular fragment, corresponding to amino acids 97-432 of Human CSK Binding Protein.
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Reacts with
(species key)
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Hu
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Specificity
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This antibody is specific for CSK Binding Protein
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at an assay dependant dilution. Use a lysis buffer made with Tris HCl (pH 8.1) and 1%laurylmaltoside (with common protease inhibitors). Use under non-reducing conditions.
Csk binding protein is an ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane microdomains (rafts), which however migrates on SDS-PAGE gels anomalously as an 80 kDa molecule. The reason is, that PAG has a pI value of about 4.6 and binds poorely SDS and therefore it had decreased mobility on SDS gels.
Is unsuitable for IP (use ab4206 or ab14989).
May be unsuitable for ICC-IF due to unsuccesful results in submitted Abreview. (Please see Abreview comments)
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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RAMOS and Jurkat cells
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Cellular localization
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Cell Membrane; Single-pass type III membrane protein. Note=Present in lipid rafts.
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Research areas
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Signal Transduction >> Adapters >> Transmembrane
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
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Relevance
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Csk binding protein, also known as PAG (phosphoprotein associated with GEMs), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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MEM-255
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Isotype
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IgG2a
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Purity
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>95% by SDS-PAGE
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Storage buffer
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Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from ascites by protein A-affinity chromatography.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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Csk-Binding Protein is also known as PAG (Protein Associated with Glycosphingolipid-enriched microdomains).
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PAG antibody [MEM-255] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PAG antibody [MEM-255] images, references, products related to ab9527 and other tools.
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PAG antibody [MEM-255] images:
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 Immunoprecipitation
1. Frozen Raji cells lysate, immunoprecipitated with ab4206
2. Fresh Raji cells lysate, immunoprecipitated with ab4206
3. Frozen Raji cells lysate, immunoprecipitated with ab14989
Reprobe with ab9527.
The immunoprecipitation used covalently bound antibody to Sepharose beads as immunosorbent. This method of precipitation obviously differs from proteinA/G-sepharose or GoatxMs -Sepharose mediated immunoprecipitation protocols.
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References for PAG antibody [MEM-255] (ab9527)
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This product has been used in: (two most recent references)
Mutch CM et al. Activation-induced endocytosis of the raft-associated transmembrane adaptor protein LAB/NTAL in B lymphocytes: evidence for a role in internalization of the B cell receptor. Int Immunol 19:19-30 (2007). PubMed: 17090619
Brdickova N et al. Interaction between two adapter proteins, PAG and EBP50: a possible link between membrane rafts and actin cytoskeleton. FEBS Lett 507:133-6 (2001). PubMed: 11684085
See all 3 publication references for this product.
If you publish research using ab9527 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to PAG
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PAG antibody [MEM-255] - more information
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