![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAG antibody [PAG-C1] (ab4206) image](#PAG-Primary-antibodies-ab4206-1.jpg)
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Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Overview
Product name
Anti-PAG antibody [PAG-C1]
See all PAG products (5) ...
Description
Mouse monoclonal [PAG-C1] to PAG
Tested applications
IHC-P, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Cow, Human
Immunogen
Synthetic C-teminal peptide (last 15 amino acids) (Human) conjugated to KLH.
Epitope
Located in the C-terminal domain.
Positive control
Human Ramos cell line, Raji cell line Mouse spleen cells
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Purification notes
Purified from hybridoma culture supernatant by protein A affinity chromatography.
Clonality
Monoclonal
Clone number
PAG-C1
Isotype
IgG2b
Research areas
Signal Transduction >> Adapters >> Transmembrane
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4206 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
IHC-P: Use at an assay dependent dilution.
IP: Use at an assay dependent concentration. Use covalently bound ab4206 to Sepharose beads as immunosorbent. This method of precipitation obviously differs from protein A/G-sepharose or Goat xMs Sepharose mediated immunoprecipitation protocols.
This antibody can be used for WB, but the signal is very faint, and we do not recommend or guarantee it for this method. We recommend the clone MEM-255 (ab9527) or the rabbit polyclonal ab14989, which are much better for WB.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling.
Tissue specificity
Ubiquitously expressed. Present in germinal center B-cells, plasma cells, T-cells, monocytes and platelets (at protein level).
Post-translational
modifications
Palmitoylated.
Phosphorylated by FYN on Tyr-317 in resting T-cells; which promotes interaction with CSK. Dephosphorylated by PTPRC/CD45 upon TCR activation; which leads to CSK dissociation. May also be dephosphorylated by PTPN11. Hyperphosphorylated in mast cells upon FCER1 activation.
Cellular localization
Cell membrane. Present in lipid rafts.
Target information above from: UniProt accessionQ9NWQ8
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CBP antibody
- Csk Binding Protein antibody
- Csk-binding protein antibody
see all
Database links
- Entrez Gene: 55824 Human
- Entrez Gene: 94212 Mouse
- Entrez Gene: 64019 Rat
- Omim: 605767 Human
- SwissProt: Q9NWQ8 Human
- SwissProt: Q3U1F9 Mouse
- SwissProt: Q9JM80 Rat
- Unigene: 266175 Human
see all
Anti-PAG antibody [PAG-C1] images:
Immunoprecipitation
1. Frozen Raji cells lysate, immunoprecipitated with ab4206
2. Fresh Raji cells lysate, immunoprecipitated with ab4206
3. Frozen Raji cells lysate, immunoprecipitated with ab14989
Reprobe with ab9527.
The immunoprecipitation used covalently bound antibody to Sepharose beads as immunosorbent. This method of precipitation obviously differs from proteinA/G-sepharose or GoatxMs -Sepharose mediated immunoprecipitation protocols.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAG antibody [PAG-C1] (ab4206)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAG antibody [PAG-C1] (ab4206)](/ps/datasheet/images/4/ab4206/PAG-Primary-antibodies-ab4206-1.jpg)
ab4206 staining PAG in human germinal center of lymphatic follicle and in dispersed T cells in appendix tissue section by immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections). Tissue underwent heat mediated antigen retrieval in citrate buffer pH 6.1. Th image show positive signal in T-cells.
References for Anti-PAG antibody [PAG-C1] (ab4206)
This product has been referenced in:
- Baumgartner Met al. Constitutive exclusion of Csk from Hck-positive membrane microdomains permits Src kinase-dependent proliferation of Theileria-transformed B lymphocytes. Blood 101:1874-81 (2003).Read more (PubMed: 12411311) »
- Vang Tet al. Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase a inhibits T cell receptor signaling. J Biol Chem 278:17597-600 (2003).Read more (PubMed: 12665526) »
See all 2 publications for this product
Publishing research using ab4206? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"