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Immunoprecipitation
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Western blot - PAG antibody (ab14989)
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Product Name
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PAG antibody
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See all PAG antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to PAG
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Immunogen
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Recombinant fragment, corresponding to amino acids 97-432 of Human PAG.
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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IHC-P, IP, WB
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Abreviews
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Application notes
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Recommended dilutions
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 51.8 kDa.
Use a lysis buffer made with Tris HCl (pH 8.1) and 1%laurylmaltoside (with common protease inhibitors), mild detergents such as NP-40 do not work. Non-denaturing conditions should be used.
IHC-P: Use at an assay dependent dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
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Human Ramos cell line, Raji cell line
Mouse spleen cells
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Cellular localization
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Cell Membrane; Single-pass type III membrane protein. Note=Present in lipid rafts.
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Research areas
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Signal Transduction >> Adapters >> Transmembrane
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
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Relevance
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Csk binding protein, also known as PAG (phosphoprotein associated with GEMs), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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>95% by SDS-PAGE
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Storage buffer
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Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from rabbit antisera by precipitation methods.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PAG antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PAG antibody images, references, products related to ab14989 and other tools.
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PAG antibody images:
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 Immunoprecipitation
1. Frozen Raji cells lysate, immunoprecipitated with ab4206
2. Fresh Raji cells lysate, immunoprecipitated with ab4206
3. Frozen Raji cells lysate, immunoprecipitated with ab14989
Reprobe with ab9527.
The immunoprecipitation used covalently bound antibody to Sepharose beads as immunosorbent. This method of precipitation obviously differs from proteinA/G-sepharose or GoatxMs -Sepharose mediated immunoprecipitation protocols.
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References for PAG antibody (ab14989)
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This product has been used in:
Boyd RS et al. Protein profiling of plasma membranes defines aberrant signaling pathways in mantle cell lymphoma. Mol Cell Proteomics : (2009). PubMed: 19346216
If you publish research using ab14989 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to PAG
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PAG antibody - more information
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Customer reviews (feedback) regarding PAG antibody |
Customer FAQs regarding PAG antibody |
Protocols for PAG antibody |
Price and availability of products related to PAG antibody |
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PAG antibody - related products:
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PAG antibody - other tools:
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Contact Abcam with a Technical Enquiry about PAG antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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