Anti-ASAP2 antibody (ab137514)
Key features and details
- Rabbit polyclonal to ASAP2
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-ASAP2 antibody
See all ASAP2 primary antibodies -
Description
Rabbit polyclonal to ASAP2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Xenopus laevis -
Immunogen
Synthetic peptide corresponding to Human ASAP2 aa 5-194 (N terminal).
Database link: O43150-2 -
Positive control
- 293T, A431, Jurkat, Raji and Mouse brain whole cell lysates.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137514 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 107 kDa.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 107 kDa. |
Target
-
Function
Activates the small GTPases ARF1, ARF5 and ARF6. Regulates the formation of post-Golgi vesicles and modulates constitutive secretion. Modulates phagocytosis mediated by Fc gamma receptor and ARF6. Modulates PXN recruitment to focal contacts and cell migration. -
Tissue specificity
Detected in heart, brain, placenta, kidney, monocytes and pancreas. -
Sequence similarities
Contains 2 ANK repeats.
Contains 1 Arf-GAP domain.
Contains 1 PH domain.
Contains 1 SH3 domain. -
Domain
The conserved Arg-464 in the Arf-GAP domain probably becomes part of the active site of bound small GTPases and is necessary for GTP hydrolysis. -
Post-translational
modificationsPhosphorylated on tyrosine residues by SRC and PTK2B. -
Cellular localization
Cytoplasm. Golgi apparatus > Golgi stack membrane. Cell membrane. Colocalizes with F-actin and ARF6 in phagocytic cups. - Information by UniProt
-
Database links
- Entrez Gene: 8853 Human
- Entrez Gene: 211914 Mouse
- Omim: 603817 Human
- SwissProt: O43150 Human
- SwissProt: Q7SIG6 Mouse
- Unigene: 555902 Human
- Unigene: 358946 Mouse
-
Alternative names
- AMAP 2 antibody
- AMAP2 antibody
- ANK repeat and PH domain-containing protein 2 antibody
see all
Images
-
All lanes : Anti-ASAP2 antibody (ab137514) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 107 kDa
7.5% SDS PAGE -
Anti-ASAP2 antibody (ab137514) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 107 kDa
7.5% SDS PAGE
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab137514 has not yet been referenced specifically in any publications.