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Products:Cardiovascular >> Blood >> Fibrinolysis / Thrombolysis
Overview
Product name
Anti-PAI1 antibody [3E529]
See all PAI1 products (19) ...
Description
Mouse monoclonal [3E529] to PAI1
Specificity
PAI1 antibody [3E529] (ab20286) recognizes both free and complexed forms of Human PAI1.
Tested applications
ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein: human PAI.
Properties
Form
Liquid
Storage instructions
Store at -80°C
Storage buffer
Preservative: None
Constituents: 50mM Sodium phosphate, 100mM Sodium chloride, 1mM EDTA. pH 6.6
Concentration
Concentration information loading...
Purity
Protein A purified
Purification notes
PAI1 antibody [3E529] (ab20286) is >98% pure (SDS-PAGE).
Clonality
Monoclonal
Clone number
3E529
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Serine protease inhibitors >> SERPINs
Cardiovascular >> Blood >> Fibrinolysis / Thrombolysis
Applications
Show applications key
Our Abpromise guarantee covers the use of ab20286 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
Target
Function
This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
Tissue specificity
Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
Involvement in disease
Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
Sequence similarities
Belongs to the serpin family.
Post-translational
modifications
Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
-Met-370 bond.
Cellular localization
Secreted.
Target information above from: UniProt accessionP05121
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Clade E antibody
- Endothelial plasminogen activator inhibitor antibody
- Nexin antibody
see all
Database links
- Entrez Gene: 5054 Human
- Omim: 173360 Human
- SwissProt: P05121 Human
- Unigene: 414795 Human
- Unigene: 713079 Human
References for Anti-PAI1 antibody [3E529] (ab20286)
ab20286 has not yet been referenced specifically in any publications.
Publishing research using ab20286? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"