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Products:Cardiovascular >> Blood >> Fibrinolysis / Thrombolysis
Overview
Product name
Anti-PAI1 antibody
See all PAI1 products (19) ...
Description
Rabbit polyclonal to PAI1
Specificity
Ab20562 recognizes Human PAI-1 both alone and in complex under reducing and native conditions.
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Rat, Human
Immunogen
Recombinant full length protein (Human PAI1).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 0.1M Sodium chloride, 0.05M Sodium phosphate, 1mM EDTA. pH 6.6
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Serine protease inhibitors >> SERPINs
Cardiovascular >> Blood >> Fibrinolysis / Thrombolysis
Applications
Show applications key
Our Abpromise guarantee covers the use of ab20562 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/50000ELISA: 1/50000
WB: 1/5000Predicted molecular weight: 45 kDa.(Dilution optimised using Chromogenic detection)
Target
Function
This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
Tissue specificity
Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
Involvement in disease
Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
Sequence similarities
Belongs to the serpin family.
Post-translational
modifications
Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
-Met-370 bond.
Cellular localization
Secreted.
Target information above from: UniProt accessionP05121
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Clade E antibody
- Endothelial plasminogen activator inhibitor antibody
- Nexin antibody
see all
Database links
- Entrez Gene: 5054 Human
- Entrez Gene: 24617 Rat
- Omim: 173360 Human
- SwissProt: P05121 Human
- SwissProt: P20961 Rat
- Unigene: 414795 Human
- Unigene: 713079 Human
- Unigene: 29367 Rat
References for Anti-PAI1 antibody (ab20562)
This product has been referenced in:
- Velusamy T & Jain SK Effects of high glucose and ketosis (acetoacetate, ss-hydroxybutyrate) on PAI-1 secretion in human umbilical vascular endothelial cells. Clin Appl Thromb Hemost 17:288-92 (2011). WB; Human.Read more (PubMed: 20530060) »
- Simón Det al. Expression of plasminogen activator inhibitor-1 by olfactory ensheathing glia promotes axonal regeneration. Glia 59:1458-71 (2011). WB; Rat.Read more (PubMed: 21626571) »
See all 3 publications for this product
Publishing research using ab20562? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"