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Anti-PAI1 antibody (ab28207)

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Overview

Product name

Anti-PAI1 antibody
See all PAI1 products (19) ...

Description

Rabbit polyclonal to PAI1

Tested applications

IHC-Fr, WB, IHC-Pmore details

Cross reactivity

Reacts with

Mouse

Immunogen

Recombinant full length PAI1 protein (Mouse).

Positive control

Paraffin sections of lung and carotid.

Properties

Form

Liquid

Storage instructions

Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 0.1M Sodium chloride, 0.05M Sodium phosphate, 1mM EDTA. pH 6.6.

Concentration

Concentration information loading...

Purity

Protein A purified

Purification notes

This is an IgG purified fraction (purified by Protein A Sepharose).

Clonality

Polyclonal

Isotype

IgG

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAI1 antibody (ab28207)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAI1 antibody (ab28207) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab28207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

IHC-P: Use at a concentration of 1 - 10 µg/ml.
IHC-Fr: Use at an assay dependent dilution (PMID 18276785).
WB: Use at a concentration of 1-5 µg/ml. Predicted molecular weight: 45 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.

Tissue specificity

Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.

Involvement in disease

Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.

Sequence similarities

Belongs to the serpin family.

Post-translational
modifications

Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
-Met-370 bond.

Cellular localization

Secreted.

Target information above from: UniProt accessionP05121 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Clade E antibody
  • Endothelial plasminogen activator inhibitor antibody
  • Nexin antibody
  • PAI 1 antibody
  • PAI antibody
  • PAI-1 antibody
  • PAI1_HUMAN antibody
  • PLANH1 antibody
  • Plasminogen activator inhibitor 1 antibody
  • Plasminogen activator inhibitor type 1 antibody
  • Serine (or cysteine) proteinase inhibitor antibody
  • Serpin E1 antibody
  • Serpin peptidase inhibitor clade E antibody
  • Serpine 1 antibody
  • Serpine1 antibody
see all

Anti-PAI1 antibody images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAI1 antibody (ab28207)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAI1 antibody (ab28207)

ab28207 staining PAI1 in E18.5 mouse tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). Subsequent to deparaffinization, the sections were hydrated, heated in 10mM citrate buffer (pH 6.0) and treated with 1% H2O2 in methanol for 10 min and blocked with 10% of normal serum. The primary antibody was incubated with sample at 4°C overnight.

Image from Xing Yiming et. al., Development 137:825-33 (2010) Fig 1C.

References for Anti-PAI1 antibody (ab28207)

This product has been referenced in:

  • Cunha SIet al. Genetic and pharmacological targeting of activin receptor-like kinase 1 impairs tumor growth and angiogenesis. J Exp Med 207:85-100, S1-5 (2010). WB; Mouse.Read more (PubMed: 20065063) »
  • Xing Yet al. Signaling via Alk5 controls the ontogeny of lung Clara cells. Development 137:825-33 (2010). IHC-P; Mouse.Read more (PubMed: 20147383) »

See all 6 publications for this product

Publishing research using ab28207? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"