Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cardiovascular >> Blood >> Fibrinolysis / Thrombolysis
Overview
Product name
Anti-PAI1 antibody
See all PAI1 products (19) ...
Description
Rabbit polyclonal to PAI1
Tested applications
IHC-Fr, WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse
Immunogen
Recombinant full length PAI1 protein (Mouse).
Positive control
Paraffin sections of lung and carotid.
Properties
Form
Liquid
Storage instructions
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 0.1M Sodium chloride, 0.05M Sodium phosphate, 1mM EDTA. pH 6.6.
Concentration
Concentration information loading...
Purity
Protein A purified
Purification notes
This is an IgG purified fraction (purified by Protein A Sepharose).
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Serine protease inhibitors >> SERPINs
Cardiovascular >> Blood >> Fibrinolysis / Thrombolysis
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAI1 antibody (ab28207)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28207 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr
WB
- 1 Image
IHC-P
Application notes
IHC-P: Use at a concentration of 1 - 10 µg/ml.
IHC-Fr: Use at an assay dependent dilution (PMID 18276785).
WB: Use at a concentration of 1-5 µg/ml. Predicted molecular weight: 45 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
Tissue specificity
Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
Involvement in disease
Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
Sequence similarities
Belongs to the serpin family.
Post-translational
modifications
Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
-Met-370 bond.
Cellular localization
Secreted.
Target information above from: UniProt accessionP05121
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Clade E antibody
- Endothelial plasminogen activator inhibitor antibody
- Nexin antibody
see all
Database links
- Entrez Gene: 18787 Mouse
- SwissProt: P22777 Mouse
- Unigene: 250422 Mouse
Anti-PAI1 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAI1 antibody (ab28207)
ab28207 staining PAI1 in E18.5 mouse tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). Subsequent to deparaffinization, the sections were hydrated, heated in 10mM citrate buffer (pH 6.0) and treated with 1% H2O2 in methanol for 10 min and blocked with 10% of normal serum. The primary antibody was incubated with sample at 4°C overnight.
Image from Xing Yiming et. al., Development 137:825-33 (2010) Fig 1C.
References for Anti-PAI1 antibody (ab28207)
This product has been referenced in:
- Cunha SIet al. Genetic and pharmacological targeting of activin receptor-like kinase 1 impairs tumor growth and angiogenesis. J Exp Med 207:85-100, S1-5 (2010). WB; Mouse.Read more (PubMed: 20065063) »
- Xing Yet al. Signaling via Alk5 controls the ontogeny of lung Clara cells. Development 137:825-33 (2010). IHC-P; Mouse.Read more (PubMed: 20147383) »
See all 6 publications for this product
Publishing research using ab28207? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"