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Products:Cardiovascular >> Blood >> Fibrinolysis / Thrombolysis
Overview
Product name
Anti-PAI1 antibody
See all PAI1 products (19) ...
Description
Rabbit polyclonal to PAI1
Tested applications
ICC, WBmore details
Cross reactivity
Reacts with
Mouse, Rat
Immunogen
Recombinant full length protein (Rat).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Serine protease inhibitors >> SERPINs
Cardiovascular >> Blood >> Fibrinolysis / Thrombolysis
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Western blot - PAI1 antibody (ab7205)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC
- 1 Image
WB
Application notes
ICC: 1/200 (PMID 19036760).
WB: 1/1000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
Tissue specificity
Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
Involvement in disease
Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
Sequence similarities
Belongs to the serpin family.
Post-translational
modifications
Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
-Met-370 bond.
Cellular localization
Secreted.
Target information above from: UniProt accessionP05121
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Clade E antibody
- Endothelial plasminogen activator inhibitor antibody
- Nexin antibody
see all
Database links
- Entrez Gene: 18787 Mouse
- Entrez Gene: 24617 Rat
- SwissProt: P22777 Mouse
- SwissProt: P20961 Rat
- Unigene: 250422 Mouse
- Unigene: 29367 Rat
Anti-PAI1 antibody images:
Western blot - PAI1 antibody (ab7205)
Anti-PAI1 antibody (ab7205) at 1/1000 dilution
Rat PAI1 detected by Western blot on Day 3 in a glomerular nephritis model.
References for Anti-PAI1 antibody (ab7205)
This product has been referenced in:
- Artaza JN & Norris KC Vitamin D reduces the expression of collagen and key profibrotic factors by inducing an antifibrotic phenotype in mesenchymal multipotent cells. J Endocrinol 200:207-21 (2009). WB, ICC; Mouse.Read more (PubMed: 19036760) »
- Artaza JNet al. Myostatin promotes a fibrotic phenotypic switch in multipotent C3H 10T1/2 cells without affecting their differentiation into myofibroblasts. J Endocrinol 196:235-49 (2008).Read more (PubMed: 18252947) »
See all 2 publications for this product
Publishing research using ab7205? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"