PAI1 + tPA Human ELISA Kit (ab108892)
- Product namePAI1 + tPA Human ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Milk, Serum, Plasma, Tissue
- Assay typeSandwich
- Sensitivity= 63 pg/ml
- Range0.063 ng/ml - 4 ng/ml
- Assay time4h 00m
- Species reactivityReacts with: Human
- Product overview
Type I plasminogen activator inhibitor (PAI-1) is a 50 kDa serpin family member that inhibits tissue- and urokinase-type plaminogen activators (t-PA, u-PA). Whereas tPA is a 68 kDa serine protease that converts the plasminogen into plasmin and facilitates the digestion of fibrin clots. In plasma, half or more of PAI-1 and most tPA present in the circulation is in an inhibited complex. In the resting state in healthy individuals, typically less than 20% of tPA is present in its free form in the plasma. In normal individuals as well as in patients with recurrent venous thrombosis, high PAI-1 plasma concentration is usually associated with high tPA antigen (but not with free tPA) levels. PAI-1/tPA complex, a novel fibrinolytic marker, increases during the pregnancy-associated hypercoagulable state, atherosclerosis and vascular spasm. Determination of PAI-1/tPA complex may provide valuable prognostic information with respect to breast cancer patients and myocardial infarction in patients with manifest coronary heart disease.
Ab108892 Human PAI-1/tPA ELISA kit is designed for detection of PAI-1/tPA in human plasma, tissue extracts or cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures PAI-1/tPA in 4 hours. A polyclonal antibody specific for PAI-1 has been pre-coated onto a microplate. PAI-1/tPA complex in standards and samples is sandwiched by the immobilized antibody against PAI-1 and a biotinylated polyclonal antibody against tPA, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Biotinylated tPA Antibody (100X) 1 x 80µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml PAI-1/tPA Microplate 1 unit PAI-1/tPA Standard 1 unit Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 1 x 30ml
- Clade EEndothelial plasminogen activator inhibitorNexin
- PAIPAI 1PAI-1PAI1_HUMANPLANH1Plasminogen activator inhibitor 1Plasminogen activator inhibitor type 1Serine (or cysteine) proteinase inhibitorSerpin E1Serpin peptidase inhibitor clade ESerpine 1Serpine1
Our Abpromise guarantee covers the use of ab108892 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for PAI1 + tPA Human ELISA Kit (ab108892)
ab108892 has not yet been referenced specifically in any publications.