Overview
- Product namePAI1 + tPA Human ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Milk, Serum, Plasma, Tissue
- Assay typeSandwich
- Sensitivity= 63 pg/ml
- Range0.063 ng/ml - 4 ng/ml
- Recovery
98.5 %
- Assay time4h 00m
- Species reactivityReacts with: Human
- Product overview
Type I plasminogen activator inhibitor (PAI-1) is a 50 kDa serpin family member that inhibits tissue- and urokinase-type plaminogen activators (t-PA, u-PA). Whereas tPA is a 68 kDa serine protease that converts the plasminogen into plasmin and facilitates the digestion of fibrin clots. In plasma, half or more of PAI-1 and most tPA present in the circulation is in an inhibited complex. In the resting state in healthy individuals, typically less than 20% of tPA is present in its free form in the plasma. In normal individuals as well as in patients with recurrent venous thrombosis, high PAI-1 plasma concentration is usually associated with high tPA antigen (but not with free tPA) levels. PAI-1/tPA complex, a novel fibrinolytic marker, increases during the pregnancy-associated hypercoagulable state, atherosclerosis and vascular spasm. Determination of PAI-1/tPA complex may provide valuable prognostic information with respect to breast cancer patients and myocardial infarction in patients with manifest coronary heart disease.
Ab108892 Human PAI-1/tPA ELISA kit is designed for detection of PAI-1/tPA in human plasma, tissue extracts or cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures PAI-1/tPA in 4 hours. A polyclonal antibody specific for PAI-1 has been pre-coated onto a microplate. PAI-1/tPA complex in standards and samples is sandwiched by the immobilized antibody against PAI-1 and a biotinylated polyclonal antibody against tPA, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsSandwich ELISA more details
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests Biotinylated tPA Antibody (100X) 1 x 80µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml PAI-1/tPA Microplate 1 unit PAI-1/tPA Standard 1 unit Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 1 x 30ml -
Research Areas
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Alternative names
- Clade EEndothelial plasminogen activator inhibitorNexin
- PAIPAI 1PAI-1PAI1_HUMANPLANH1Plasminogen activator inhibitor 1Plasminogen activator inhibitor type 1Serine (or cysteine) proteinase inhibitorSerpin E1Serpin peptidase inhibitor clade ESerpine 1Serpine1
see all
- Entrez Gene: 5327 Human
- Entrez Gene: 5054 Human
- SwissProt: P05121 Human
- SwissProt: P00750 Human
Applications
Our Abpromise guarantee covers the use of ab108892 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Sandwich ELISA | sELISA |
Protocols
References for PAI1 + tPA Human ELISA Kit (ab108892)
ab108892 has not yet been referenced specifically in any publications.
