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Anti-PAIP2 antibody (ab33455)

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Overview

Product name

Anti-PAIP2 antibody
See all PAIP2 products (2) ...

Description

Rabbit polyclonal to PAIP2

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Chicken, Cow

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human PAIP2.

Read Abcam's proprietary immunogen policy

(Peptide available as ab36582.)

Positive control

This antibody gave a positive signal in the following whole cell lysates: Jurkat; A431; HEK293; SHSY-5Y.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation

  • Western blot - PAIP2 antibody (ab33455)Western blot - PAIP2 antibody (ab33455) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab33455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 1 µg/mlDetects a band of approximately 17 kDa (predicted molecular weight: 14.9 kDa).

Target

Relevance

PAIP2 acts as a repressor in the regulation of translation initiation of poly(A)-containing mRNAs. Its inhibitory activity on translation is mediated via its action on PABPC1. It displaces the interaction of PABPC1 with poly(A) RNA and competes with PAIP1 for binding to PABPC1. Its association with PABPC1 results in disruption of the cytoplasmic poly(A) RNP structure organization (referenced from Swiss-Prot).

Cellular localization

Cytoplasmic

Alternative names

  • PABP-interacting protein 2 antibody
  • poly(A) binding protein interacting protein 2 antibody

Database links

see all

Anti-PAIP2 antibody images:

  Western blot - PAIP2 antibody (ab33455)

Western blot - PAIP2 antibody (ab33455)

All lanes : Anti-PAIP2 antibody (ab33455) at 1 µg/ml

Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
Lane 4 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Performed under reducing conditions.

Predicted band size : 14.9 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?)

References for Anti-PAIP2 antibody (ab33455)

ab33455 has not yet been referenced specifically in any publications.

Publishing research using ab33455? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"