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- PAK1 + PAK2 + PAK3 (phospho S141) antibody (ab5247)
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- PAK1 + PAK2 + PAK3 (phospho S141) antibody (ab5247)
(enlarge)
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Product Name
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PAK1 + PAK2 + PAK3 (phospho S141) antibody
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See all PAK1 + PAK2 + PAK3 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to PAK1 + PAK2 + PAK3 (phospho S141)
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Immunogen
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Chemically synthesized phosphopeptide derived from a region of human PAK2 that contains serine 141. The sequence is conserved in rat and rabbit.
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Reacts with
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Hu
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Predicted to react
(species key)
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Rat, Rb
(Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 0.1 - 1.0 µg/ml. Predicted molecular weight: 65 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Cos cells transiently transfected with PAK 1, PAK 2, or PAK 3, and co transfected with V12 Cdc42 and grown either as adherent cultures to activate PAK, or placed in suspension to suppress PAK activation.
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Cellular localization
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Cytoplasm. Recruited to focal adhesions upon activation
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Research areas
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Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Focal Adhesions
Cell Biology >> Apoptosis >> Intracellular >> Kinases
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Relevance
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In mammals, there are several identified isoforms of p21 Activated Protein Kinases or PAKs: PAK1 and PAK3 are mostly brain specific, while PAK2 is expressed ubiquitously. Mutations of the gene coding for PAK3 are associated with X linked mental retardation and PAK3 is a key regulator of synapse formation and plasticity in the hippocampus. PAK3 is thought to play a key role in regulation of cell shape and motility as well as cell death.
P21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK 1, PAK 2, and PAK 3 (also known as alpha PAK, gamma PAK, and beta PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase mediated proteolytic cleavage. Autophosphorylation of serine 141 (serine 144 for PAK 1 and serine 139 PAK 3), catalyzed by Cdc42, is required for activation of PAK.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: PBS, 1mg/ml BSA (IgG, protease free). pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PAK. The final product is generated by affinity chromatography using a PAK 2 derived peptide that is phosphorylated at serine 141.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PAK1 + PAK2 + PAK3 (phospho S141) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PAK1 + PAK2 + PAK3 (phospho S141) antibody images, references, products related to ab5247 and other tools.
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PAK1 + PAK2 + PAK3 (phospho S141) antibody images:
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 - PAK1 + PAK2 + PAK3 (phospho S141) antibody (ab5247)
Predicted band size : 65 kDa
Induction of Activation: Extracts prepared from Cos cells transiently transfected with PAK 1 and V12 Cdc42, and kept in suspension (inactive) or as adherent cultures (active) were resolved by SDSPAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was either not treated or treated with lambda phosphatase, blocked with a 5% BSA-TBST buffer overnight at 4°C, and then incubated with 0.50 µg/mL ab5247 antibody for two hours at room temperature in a 3% BSA-TBST buffer. After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method.
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- PAK1 + PAK2 + PAK3 (phospho S141) antibody (ab5247)
Predicted band size : 65 kDa
Peptide Competition: Extracts prepared from Cos cells transiently transfected with PAK 1 (lanes 1-4), PAK 2 (lanes 5-8), or PAK 3 (lanes 9-12) and cotransfected with V12 Cdc42 to induce activation were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.50 µg/mL ab5247 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 5, 9), the nonphosphopeptide corresponding to the immunogen (2, 6, 10), a generic phosphoserine containing peptide (3, 7, 11), or, the phosphopeptide immunogen (4, 8, 12). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that the ab5247 antibody is highly selective for the phosphorylated form of PAK, and that the antibody can be used to detect an active form of PAK 1 as well as PAK 2 and PAK 3.
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Search PubMed (MEDLINE) for references to PAK1 + PAK2 + PAK3
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