PAK1 + PAK2 + PAK3 (phospho S144 + 141 + 139) antibody (ab63513)

Product Name 

PAK1 + PAK2 + PAK3 (phospho S144 + 141 + 139) antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to PAK1 + PAK2 + PAK3 (phospho S144 + 141 + 139)

Immunogen 

A synthesized phosphopeptide derived from human PAK1 + PAK2 + PAK3 around the phosphorylation site of serine 144 + 141 + 139

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

This antibody detects endogenous levels of PAK1 + PAK2 + PAK3 only when phosphorylated at serine 144 + 141 + 139.

Tested applications 
(see key)

ELISA, IHC-P

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/10000.
IHC-P: 1/50 - 1/100.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human brain tissue sections

Cellular localization 

Cytoplasm. Recruited to focal adhesions upon activation

Research areas 

Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Focal Adhesions
Cell Biology >> Apoptosis >> Intracellular >> Kinases

Relevance 

In mammals, there are several identified isoforms of p21 Activated Protein Kinases or PAKs: PAK1 and PAK3 are mostly brain specific, while PAK2 is expressed ubiquitously. Mutations of the gene coding for PAK3 are associated with X linked mental retardation and PAK3 is a key regulator of synapse formation and plasticity in the hippocampus. PAK3 is thought to play a key role in regulation of cell shape and motility as well as cell death.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

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