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All lanes : Anti-PAK1 + PAK2 + PAK3 (phospho T402) antibody (ab30577) at 1/1000 dilution
Lane 1 : untreated rat hippocampal lysate
Lane 2 : rat hippocampal lysate, pretreated with 1200 units lambda Ptase for 30 min (negative control)
Predicted band size : 60 kDa
Observed band size : 68 kDa (why is the actual band size different from the predicted?)
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