PAK1 + PAK2 + PAK3 (phospho T402) antibody (ab30577)

Product Name 

PAK1 + PAK2 + PAK3 (phospho T402) antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to PAK1 + PAK2 + PAK3 (phospho T402)

Immunogen 

Synthetic Phosphopeptide corresponding to amino acid residues surrounding the phospho Thr402 of rat p21 Activated Kinase 2 (PAK2). The peptide sequence used is identical in PAK1, 2 and 3.

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

ab30577 recognises PAK1 + PAK2 + PAK3 (phospho T402).

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 60 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Rat hippocampal lysate

Cellular localization 

Cytoplasm. Recruited to focal adhesions upon activation

Research areas 

Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Focal Adhesions
Cell Biology >> Apoptosis >> Intracellular >> Kinases

Relevance 

In mammals, there are several identified isoforms of p21 Activated Protein Kinases or PAKs: PAK1 and PAK3 are mostly brain specific, while PAK2 is expressed ubiquitously. Mutations of the gene coding for PAK3 are associated with X linked mental retardation and PAK3 is a key regulator of synapse formation and plasticity in the hippocampus. PAK3 is thought to play a key role in regulation of cell shape and motility as well as cell death.

Autophosphorylation of Thr402 in the protein has been found to be essential for activation of PAK.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: None
Constituents: 50% Glycerol, 0.1mg/ml BSA, 150mM Sodium chloride, 10mM HEPES. pH 7.5

Material safety datasheet (MSDS) for this product:
Glycerol MSDS

Purification notes 

ab30577 was purified by sequential chromatography on phospho and dephosphopeptide affinity columns.

Form 

Liquid

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Note: Thr402 in PAK2 corresponds to Thr423 in human PAK1.

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