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Products:Cell Biology >> Apoptosis >> Intracellular >> Kinases
Overview
Product name
Anti-PAK1 + PAK2 + PAK3 (phospho T402) antibody
See all PAK1 + PAK2 + PAK3 products (8) ...
Description
Rabbit polyclonal to PAK1 + PAK2 + PAK3 (phospho T402)
Specificity
ab30577 recognises PAK1 + PAK2 + PAK3 (phospho T402).
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic Phosphopeptide corresponding to amino acid residues surrounding the phospho Thr402 of rat p21 Activated Kinase 2 (PAK2). The peptide sequence used is identical in PAK1, 2 and 3.
Positive control
Rat hippocampal lysate
General notes
Note: Thr402 in PAK2 corresponds to Thr423 in human PAK1.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, 0.1mg/ml BSA, 150mM Sodium chloride, 10mM HEPES. pH 7.5
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab30577 was purified by sequential chromatography on phospho and dephosphopeptide affinity columns.
Primary antibody notes
Autophosphorylation of Thr402 in the protein has been found to be essential for activation of PAK.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Focal Adhesions
Cell Biology >> Apoptosis >> Intracellular >> Kinases
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Western blot - PAK1 + PAK2 + PAK3 (phospho T402) antibody (ab30577)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab30577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Detects a band of approximately 68 kDa (predicted molecular weight: 60 kDa).
Target
Relevance
In mammals, there are several identified isoforms of p21 Activated Protein Kinases or PAKs: PAK1 and PAK3 are mostly brain specific, while PAK2 is expressed ubiquitously. Mutations of the gene coding for PAK3 are associated with X linked mental retardation and PAK3 is a key regulator of synapse formation and plasticity in the hippocampus. PAK3 is thought to play a key role in regulation of cell shape and motility as well as cell death.
Cellular localization
Cytoplasm. Recruited to focal adhesions upon activation
Alternative names
- Alpha PAK antibody
- Beta PAK antibody
- Gamma PAK antibody
see all
Database links
- Entrez Gene: 5058 Human
- Entrez Gene: 5062 Human
- Entrez Gene: 5063 Human
- Entrez Gene: 18479 Mouse
- Entrez Gene: 18481 Mouse
- Entrez Gene: 224105 Mouse
- Entrez Gene: 29431 Rat
- Entrez Gene: 29432 Rat
see all
Anti-PAK1 + PAK2 + PAK3 (phospho T402) antibody images:
Western blot - PAK1 + PAK2 + PAK3 (phospho T402) antibody (ab30577)
All lanes : Anti-PAK1 + PAK2 + PAK3 (phospho T402) antibody (ab30577) at 1/1000 dilution
Lane 1 : untreated rat hippocampal lysate
Lane 2 : rat hippocampal lysate, pretreated with 1200 units lambda Ptase for 30 min (negative control)
Predicted band size : 60 kDa
Observed band size : 68 kDa (why is the actual band size different from the predicted?)
References for Anti-PAK1 + PAK2 + PAK3 (phospho T402) antibody (ab30577)
ab30577 has not yet been referenced specifically in any publications.
Publishing research using ab30577? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"