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Products:Cell Biology >> Apoptosis >> Intracellular >> Kinases
Overview
Product name
Anti-PAK1 + PAK2 + PAK3 (phospho T423 + T402 + T421) antibody
See all PAK1 + PAK2 + PAK3 products (8) ...
Description
Rabbit polyclonal to PAK1 + PAK2 + PAK3 (phospho T423 + T402 + T421)
Specificity
Detects endogenous levels of PAK1, PAK2 and PAK3 only when phosphorylated at threonine 423, threonine 402 and threonine 421 respectively (Human: Thr423/402/421; Mouse: Thr423/402/421; Rat: Thr422/402/421).
Tested applications
IHC-P, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic phosphopeptide derived from human PAK1 + PAK2 + PAK3 around the phosphorylation sites of threonine 423, threonine 402 and threonine 421 respectively (R-S-TP-M-V).
Positive control
Breast carcinoma tissue.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Focal Adhesions
Cell Biology >> Apoptosis >> Intracellular >> Kinases
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Immunocytochemistry/ Immunofluorescence - PAK1 + PAK2 + PAK3 (phospho T423) antibody (ab62155)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab62155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P
IHC-P: 1/50 - 1/100.IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/50000ELISA: 1/50000
Target
Relevance
In mammals, there are several identified isoforms of p21 Activated Protein Kinases or PAKs: PAK1 and PAK3 are mostly brain specific, while PAK2 is expressed ubiquitously. Mutations of the gene coding for PAK3 are associated with X linked mental retardation and PAK3 is a key regulator of synapse formation and plasticity in the hippocampus. PAK3 is thought to play a key role in regulation of cell shape and motility as well as cell death.
Cellular localization
Cytoplasm. Recruited to focal adhesions upon activation
Alternative names
- Alpha PAK antibody
- AlphaPAK antibody
- Beta PAK antibody
see all
Database links
- Entrez Gene: 5058 Human
- Entrez Gene: 5062 Human
- Entrez Gene: 5063 Human
- Entrez Gene: 18479 Mouse
- Entrez Gene: 18481 Mouse
- Entrez Gene: 224105 Mouse
- Entrez Gene: 29431 Rat
- Entrez Gene: 29432 Rat
see all
Anti-PAK1 + PAK2 + PAK3 (phospho T423 + T402 + T421) antibody images:
Immunocytochemistry/ Immunofluorescence - PAK1 + PAK2 + PAK3 (phospho T423) antibody (ab62155)
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using PAK1 + PAK2 + PAK3 (phospho T423) antibody (ab62155) at 1/50 - 1/100 dilution, in the presence (right panel) and absence (left panel) of immunising phosphopeptide.
References for Anti-PAK1 + PAK2 + PAK3 (phospho T423 + T402 + T421) antibody (ab62155)
ab62155 has not yet been referenced specifically in any publications.
Publishing research using ab62155? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"