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Products:Cell Biology >> Apoptosis >> Intracellular >> Kinases
Overview
Product name
Anti-PAK1 + PAK2 + PAK3 (phospho T423) antibody
See all PAK1 + PAK2 + PAK3 products (8) ...
Description
Rabbit polyclonal to PAK1 + PAK2 + PAK3 (phospho T423)
Specificity
This phospho specific polyclonal antibody is specific for phosphorylated Threonine 423 of Human PAK 1 + 2 + 3. Reactivity with non-phosphorylated PAK 1 + 2 + 3 is less than 1% by ELISA.
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: EQSKRSTpMVGTPY conjugated to KLH, corresponding to amino acids 413-433 of Human PAK 1, 408-428 of human PAK 2 and 411-431 of human PAK 3.
EQSKRSTpMV GTPY
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
This antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.
Primary antibody notes
P21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK 1, PAK 2, and PAK 3 (also known as alpha PAK, gamma PAK, and beta PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase mediated proteolytic cleavage. Autophosphorylation of threonine 423 (threonine 402 for PAK 2 and threonine 421 for PAK 3), catalyzed by Cdc42, is required for activation of PAK.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Focal Adhesions
Cell Biology >> Apoptosis >> Intracellular >> Kinases
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Western blot - PAK1 + PAK2 + PAK3 (phospho T423) antibody (ab2477)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2477 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/4000 - 1/20000.ELISA: 1/4000 - 1/20000.
WB: 1/500 - 1/2000.Detects a band of approximately 65 kDa.
Target
Relevance
In mammals, there are several identified isoforms of p21 Activated Protein Kinases or PAKs: PAK1 and PAK3 are mostly brain specific, while PAK2 is expressed ubiquitously. Mutations of the gene coding for PAK3 are associated with X linked mental retardation and PAK3 is a key regulator of synapse formation and plasticity in the hippocampus. PAK3 is thought to play a key role in regulation of cell shape and motility as well as cell death.
Cellular localization
Cytoplasm. Recruited to focal adhesions upon activation
Alternative names
- Alpha PAK antibody
- Beta PAK antibody
- Gamma PAK antibody
see all
Database links
- Entrez Gene: 5058 Human
- Entrez Gene: 5062 Human
- Entrez Gene: 5063 Human
- Omim: 300142 Human
- Omim: 602590 Human
- Omim: 605022 Human
- SwissProt: O75914 Human
- SwissProt: Q13153 Human
see all
Anti-PAK1 + PAK2 + PAK3 (phospho T423) antibody images:
Western blot - PAK1 + PAK2 + PAK3 (phospho T423) antibody (ab2477)
Western blot probing with PAK1 antibody (ab2477). The major band is at 65 kDa and weaker bands can be detected at 90 and 100 kDa. This picture was kindly submitted by Sue Thomas as part of her review.
References for Anti-PAK1 + PAK2 + PAK3 (phospho T423) antibody (ab2477)
ab2477 has not yet been referenced specifically in any publications.
Publishing research using ab2477? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"