PAK1 antibody [EP656Y] (ab40795)

Product Name 

PAK1 antibody [EP656Y]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [EP656Y] to PAK1

Immunogen 

Synthetic peptide corresponding to residues in the human PAK1 protein.

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

ab40795 recognises p21-activated kinase 1 (PAK1).

Tested applications 
(see key)

ICC/IF, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC: 1/250 - 1/500.
IHC-P: 1/250 - 1/500.
IP: 1/50.
WB: 1/10000 - 1/50000. Detects a band of approximately 66 kDa (predicted molecular weight: 65 kDa).

Is unsuitable for FACS.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasm. Recruited to focal adhesions upon activation.

Research areas 

Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Cell Biology >> Apoptosis >> Intracellular >> Kinases

Relevance 

The serine/threonine kinase Pak1 is an effector of small Rho GTPases, Rac1 and Cdc42. Pak1 complex specifically with Rac1 and Cdc42 in their active GTP bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. It plays an important role in the regulation of cell morphogenesis, motility, mitosis, and angiogenesis and has been implicated in the progression of many cancers.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

EP656Y

Isotype 

IgG

Purity 

IgG fraction

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

See below for PAK1 antibody [EP656Y] images, references, products related to ab40795 and other tools.

PAK1 antibody [EP656Y] images:

References for PAK1 antibody [EP656Y] (ab40795)

  This product has been used in: (two most recent references)

Long W et al. SRC-3Delta4 mediates the interaction of EGFR with FAK to promote cell migration. Mol Cell 37:321-32 (2010). WB, ICC/IF; Mouse. PubMed: 20159552

Kim SH et al. Down-regulation of p21-activated kinase 1 by progestin and its increased expression in the eutopic endometrium of women with endometriosis. Hum Reprod 24:1133-41 (2009). PubMed: 19168872

See all 5 publication references for this product.


If you publish research using ab40795 please let us know so that we can cite the reference on this datasheet.

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