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Anti-PAK1 antibody [EP795Y] (ab40852)

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Overview

Product name

Anti-PAK1 antibody [EP795Y]
See all PAK1 products (17) ...

Description

Rabbit monoclonal [EP795Y] to PAK1

Tested applications

Flow Cyt, IP, WB, IHC-P, ICC/IFmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide corresponding to residues in the N-terminus of human PAK1 protein.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Purity

Tissue culture supernatant

Purification notes

This product has not been purified.

Clonality

Monoclonal

Clone number

EP795Y

Isotype

IgG

Research areas

Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Cell Biology >> Apoptosis >> Intracellular >> Kinases

  • Western blot - PAK1 antibody [EP795Y] (ab40852)Western blot - PAK1 antibody [EP795Y] (ab40852) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - PAK1 antibody [EP795Y] (ab40852)Immunohistochemistry (Paraffin-embedded sections) - PAK1 antibody [EP795Y] (ab40852) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - PAK1 antibody [EP795Y] (ab40852)Immunocytochemistry/ Immunofluorescence - PAK1 antibody [EP795Y] (ab40852) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab40852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Flow Cyt

     Flow Cyt: 1/10

    Flow Cyt: 1/10

  • IP

     IP: 1/50

    IP: 1/50

  • ShowHide1 Image

    WB

     WB: 1/500Predicted molec...Read more →

    WB: 1/500Predicted molecular weight: 66 kDa.

  • 1 Image

    IHC-P

     IHC-P: 1/100 - 1/250.

    IHC-P: 1/100 - 1/250.

  • 1 Image

    ICC/IF

     ICC/IF: 1/50 - 1/100.

    ICC/IF: 1/50 - 1/100.

Target

Function

The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.

Sequence similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain.

Post-translational
modifications

Autophosphorylated when activated by CDC42/p21 and RAC1.

Cellular localization

Cytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation.

Target information above from: UniProt accessionQ13153 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Alpha PAK antibody
  • Alpha-PAK antibody
  • MGC130000 antibody
  • MGC130001 antibody
  • p21 activated kinase 1 antibody
  • p21-activated kinase 1 antibody
  • p21/Cdc42/Rac1 activated kinase 1 antibody
  • p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) antibody
  • p65 PAK antibody
  • p65-PAK antibody
  • PAK alpha antibody
  • PAK-1 antibody
  • Pak1 antibody
  • PAK1_HUMAN antibody
  • Paka antibody
  • PAKalpha antibody
  • Protein kinase MUK2 antibody
  • Rac/p21-activated kinase antibody
  • Serine/threonine-protein kinase PAK 1 antibody
  • STE20 homolog (yeast) antibody
see all

Database links

see all

Anti-PAK1 antibody [EP795Y] images:

  Western blot - PAK1 antibody [EP795Y] (ab40852)

Western blot - PAK1 antibody [EP795Y] (ab40852)



Predicted band size : 66 kDa
Observed band size : 66 kDa


Western blot analysis of anti- PAK1 (N-term) ab40852, dilution 1:500

A: 10ug Hela

B: 10ug A431

C: 10ug Jurkat

D: 10ug K562

E: 10ug MCF7

F: 10ug Daudi

G: 10ug NIH 3T3

H: 10ug PC12

  Immunohistochemistry (Paraffin-embedded sections) - PAK1 antibody [EP795Y] (ab40852)

Immunohistochemistry (Paraffin-embedded sections) - PAK1 antibody [EP795Y] (ab40852)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab40852.

  Immunocytochemistry/ Immunofluorescence - PAK1 antibody [EP795Y] (ab40852)

Immunocytochemistry/ Immunofluorescence - PAK1 antibody [EP795Y] (ab40852)

Immunofluorescent staining of HeLa cells using ab40852.

References for Anti-PAK1 antibody [EP795Y] (ab40852)

ab40852 has not yet been referenced specifically in any publications.

Publishing research using ab40852? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"