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ab1178 at a 1:100 dilution staining ~ 65 kDa p65 PAK in 10µg of HEK 293 cell lysate by western blot. ECL detection.
Anti-PAK1 antibody (ab1178) at 1/1000 dilution +
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 10 seconds
0
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