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Anti-PAK1 antibody (ab1178)

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Overview

Product name

Anti-PAK1 antibody
See all PAK1 products (17) ...

Description

Rabbit polyclonal to PAK1

Specificity

Displays some cross-reactivity with PAK2.

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Immunogen

Synthetic peptide: dvekrgsakellqhpflkl, corresponding to amino acids 484-502 of p65 PAK.

dvekrgsake llqhpflkl

Positive control

HEK 293 whole cell lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Western blot - PAK1 antibody (ab1178)Western blot - PAK1 antibody (ab1178) image (enlarge)

  • Western blot - Anti-PAK1 antibody (ab1178)Western blot - Anti-PAK1 antibody (ab1178) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab1178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

Reactive with p65PAK from diverse tissue sources by Western blotting.
Used at 1:100 with cell extracts.
Other immunodetection procedures not tested.

Target

Function

The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.

Sequence similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain.

Post-translational
modifications

Autophosphorylated when activated by CDC42/p21 and RAC1.

Cellular localization

Cytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation.

Target information above from: UniProt accessionQ13153 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Alpha PAK antibody
  • Alpha-PAK antibody
  • CB422 antibody
  • Gamma PAK antibody
  • hPAK65 antibody
  • MGC130000 antibody
  • MGC130001 antibody
  • p21 activated kinase 1 antibody
  • p21-activated kinase 1 antibody
  • p21/Cdc42/Rac1 activated kinase 1 antibody
  • p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) antibody
  • p21CDKN1A activated kinase 2 antibody
  • p65 PAK antibody
  • p65-PAK antibody
  • p65PAK antibody
  • PAK alpha antibody
  • PAK-1 antibody
  • Pak1 antibody
  • PAK1_HUMAN antibody
  • PAK65 antibody
  • Paka antibody
  • PAKalpha antibody
  • PAKgamma antibody
  • Protein kinase MUK2 antibody
  • Rac/p21-activated kinase antibody
  • S6 H4 kinase antibody
  • Serine threonine protein kinase PAK 2 antibody
  • Serine/threonine-protein kinase PAK 1 antibody
  • STE20 homolog (yeast) antibody
see all

Anti-PAK1 antibody images:

  Western blot - PAK1 antibody (ab1178)

Western blot - PAK1 antibody (ab1178)

ab1178 at a 1:100 dilution staining ~ 65 kDa p65 PAK in 10µg of HEK 293 cell lysate by western blot. ECL detection.

  Western blot - Anti-PAK1 antibody (ab1178)

Western blot - Anti-PAK1 antibody (ab1178)

Anti-PAK1 antibody (ab1178) at 1/1000 dilution + PAK1 protein (Tagged) (ab67946) at 0.01 µg

Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Exposure time : 10 seconds

References for Anti-PAK1 antibody (ab1178)

ab1178 has not yet been referenced specifically in any publications.

Publishing research using ab1178? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"