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Products:Cell Biology >> Apoptosis >> Intracellular >> Kinases
Overview
Product name
Anti-PAK1 antibody
See all PAK1 products (17) ...
Description
Rabbit polyclonal to PAK1
Specificity
Displays some cross-reactivity with PAK2.
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: dvekrgsakellqhpflkl, corresponding to amino acids 484-502 of p65 PAK.
dvekrgsake llqhpflkl
Positive control
HEK 293 whole cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Cell Biology >> Apoptosis >> Intracellular >> Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab1178 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 2 Images
WB
Application notes
Reactive with p65PAK from diverse tissue sources by Western blotting.
Used at 1:100 with cell extracts.
Other immunodetection procedures not tested.
Target
Function
The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain.
Post-translational
modifications
Autophosphorylated when activated by CDC42/p21 and RAC1.
Cellular localization
Cytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation.
Target information above from: UniProt accessionQ13153
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Alpha PAK antibody
- Alpha-PAK antibody
- CB422 antibody
see all
Database links
- Entrez Gene: 5058 Human
- Omim: 602590 Human
- SwissProt: Q13153 Human
- Unigene: 435714 Human
Anti-PAK1 antibody images:
Western blot - PAK1 antibody (ab1178)
ab1178 at a 1:100 dilution staining ~ 65 kDa p65 PAK in 10µg of HEK 293 cell lysate by western blot. ECL detection.
Western blot - Anti-PAK1 antibody (ab1178)
Anti-PAK1 antibody (ab1178) at 1/1000 dilution + PAK1 protein (Tagged) (ab67946) at 0.01 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 10 seconds
References for Anti-PAK1 antibody (ab1178)
ab1178 has not yet been referenced specifically in any publications.
Publishing research using ab1178? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"