PAK1 antibody (ab2551)

Product Name 

PAK1 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to PAK1

Immunogen 

E.coli expressed human PAK 1 (amino acids 333-545).

Reacts with 
(species key)

Hu

Tested applications 
(see key)

IP, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
IP: Use at a concentration of 10 - 20 µg/ml.
WB: Use at a concentration of 1.0 µg/ml.

In Western blot, this antibody detects a band of approximately 80 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasm. Recruited to focal adhesions upon activation.

Research areas 

Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Cell Biology >> Apoptosis >> Intracellular >> Kinases

Relevance 

The serine/threonine kinase Pak1 is an effector of small Rho GTPases, Rac1 and Cdc42. Pak1 complex specifically with Rac1 and Cdc42 in their active GTP bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. It plays an important role in the regulation of cell morphogenesis, motility, mitosis, and angiogenesis and has been implicated in the progression of many cancers.

Human p21-GTPase-activated Protein Kinase 1 (PAK 1) is a functional homolog of STE20 of Saccharomyces cerevisiae. PAK1 is a downstream effector of the Rho-family small GTPases Rac and Cdc42. A serine/threonine kinase, PAK 1 induces the formation of filopodia and lamellipodia in Swiss 3T3 cells. PAK 1 inhibits myosin light chain kinase, and appears to play a role in the regulation of apoptosis by phosphorylating the death agonist Bad. Membrane targeting of PAK 1 induces neurite outgrowth. PAK 1 may also regulate NFkappaB activation.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

PBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.200 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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