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Products:Cell Biology >> Apoptosis >> Intracellular >> Kinases
Overview
Product name
Anti-PAK1 antibody
See all PAK1 products (17) ...
Description
Rabbit polyclonal to PAK1
Tested applications
IP, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
E.coli expressed human PAK 1 (amino acids 333-545).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Primary antibody notes
Human p21-GTPase-activated Protein Kinase 1 (PAK 1) is a functional homolog of STE20 of Saccharomyces cerevisiae. PAK1 is a downstream effector of the Rho-family small GTPases Rac and Cdc42. A serine/threonine kinase, PAK 1 induces the formation of filopodia and lamellipodia in Swiss 3T3 cells. PAK 1 inhibits myosin light chain kinase, and appears to play a role in the regulation of apoptosis by phosphorylating the death agonist Bad. Membrane targeting of PAK 1 induces neurite outgrowth. PAK 1 may also regulate NFkappaB activation.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Cell Biology >> Apoptosis >> Intracellular >> Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2551 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP
WB
Application notes
IP: Use at a concentration of 10 - 20 µg/ml.
WB: Use at a concentration of 1.0 µg/ml.
In Western blot, this antibody detects a band of approximately 80 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain.
Post-translational
modifications
Autophosphorylated when activated by CDC42/p21 and RAC1.
Cellular localization
Cytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation.
Target information above from: UniProt accessionQ13153
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Alpha PAK antibody
- Alpha-PAK antibody
- MGC130000 antibody
see all
Database links
- Entrez Gene: 5058 Human
- Omim: 602590 Human
- SwissProt: Q13153 Human
- Unigene: 435714 Human
References for Anti-PAK1 antibody (ab2551)
ab2551 has not yet been referenced specifically in any publications.
Publishing research using ab2551? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"