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Products:Cell Biology >> Apoptosis >> Intracellular >> Kinases
Overview
Product name
Anti-PAK1 antibody
See all PAK1 products (17) ...
Description
Mouse monoclonal to PAK1
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: APRPEHTKSV YTRSVIEPLP VTPTRDVATS PISPTENNTT PPDALTRNTE KQKKKPKMSD EEILEKLRSI VSVGDPKKKY TRFEKIGQGA , corresponding to amino acids 191-280 of Human PAK1
APRPEHTKSV YTRSVIEPLP VTPTRDVATS PISPTENNTT PPDALTRNTE KQKKKPKMSD EEILEKLRSI VSVGDPKKKY TRFEKIGQGA
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG1
Light chain type
kappa
Research areas
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Cell Biology >> Apoptosis >> Intracellular >> Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab58263 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
WB: Use at a concentration of 1-5 µg/ml.
IHC-P: Use at a concentration of 3 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain.
Post-translational
modifications
Autophosphorylated when activated by CDC42/p21 and RAC1.
Cellular localization
Cytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation.
Target information above from: UniProt accessionQ13153
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Alpha PAK antibody
- Alpha-PAK antibody
- MGC130000 antibody
see all
Database links
- Entrez Gene: 5058 Human
- Omim: 602590 Human
- SwissProt: Q13153 Human
- Unigene: 435714 Human
Anti-PAK1 antibody images:
IHC-P - PAK1 antibody (ab58263)
PAK1 antibody (ab58263) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human prostate.
Western blot - PAK1 antibody (ab58263)
Western blot against tagged recombinant protein immunogen using ab58263 PAK1 antibody at 1ug/ml. Predicted band size of immunogen is 36 kDa
References for Anti-PAK1 antibody (ab58263)
ab58263 has not yet been referenced specifically in any publications.
Publishing research using ab58263? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"