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Products:Cell Biology >> Apoptosis >> Intracellular >> Kinases
Overview
Product name
Anti-PAK1 (phospho T212) antibody
See all PAK1 products (17) ...
Description
Rabbit polyclonal to PAK1 (phospho T212)
Specificity
ab75599 detects endogenous levels of PAK1 only when phosphorylated at threonine 212.
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic phosphopeptide derived from human PAK1 around the phosphorylation site of threonine 212 (P-V-TP-P-T).
Positive control
Extracts from 293 cells treated with forskolin (40µM, 30min). Human breast carcinoma tissue.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab75599 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against the non-phosphopeptide was removed by chromatography using a non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Cell Biology >> Apoptosis >> Intracellular >> Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab75599 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Predicted molecular weight: 61 kDa.
IHC-P: 1/50 - 1/100.
Target
Function
The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain.
Post-translational
modifications
Autophosphorylated when activated by CDC42/p21 and RAC1.
Cellular localization
Cytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation.
Target information above from: UniProt accessionQ13153
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Alpha PAK antibody
- Alpha-PAK antibody
- AW045634 antibody
see all
Database links
- Entrez Gene: 5058 Human
- Entrez Gene: 18479 Mouse
- Entrez Gene: 29431 Rat
- Omim: 602590 Human
- SwissProt: Q13153 Human
- SwissProt: O88643 Mouse
- SwissProt: P35465 Rat
- Unigene: 435714 Human
see all
Anti-PAK1 (phospho T212) antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAK1 (phospho T212) antibody (ab75599)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab75599, at 1/50 dilution, in the absence (left panel) and presence (right panel) of the immunising phosphopeptide.
Western blot - PAK1 (phospho T212) antibody (ab75599)
All lanes : Anti-PAK1 (phospho T212) antibody (ab75599) at 1/500 dilution
Lane 1 : Extracts from 293 cells (untreated)
Lane 2 : Extracts from 293 cells treated with forskolin (40µM, 30min),
Predicted band size : 61 kDa
Observed band size : 65 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - PAK1 (phospho T212) antibody (ab75599)
ab75599 at 1/100 dilution staining PAK1 (phospho T212) in Hela cells by ICC/IF (MeOH fixation).
References for Anti-PAK1 (phospho T212) antibody (ab75599)
ab75599 has not yet been referenced specifically in any publications.
Publishing research using ab75599? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"