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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAK1 (phospho T212) antibody (ab75599)
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Western blot - PAK1 (phospho T212) antibody (ab75599)
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Immunocytochemistry/ Immunofluorescence - PAK1 (phospho T212) antibody (ab75599)
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Product Name
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PAK1 (phospho T212) antibody
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See all PAK1 antibodies (17)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to PAK1 (phospho T212)
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Immunogen
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Synthetic phosphopeptide derived from human PAK1 around the phosphorylation site of threonine 212 (P-V-TP-P-T).
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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ab75599 detects endogenous levels of PAK1 only when phosphorylated at threonine 212.
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Tested applications
(see key)
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ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/100 - 1/200.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Predicted molecular weight: 61 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Extracts from 293 cells treated with forskolin (40µM, 30min). Human breast carcinoma tissue.
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Cellular localization
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Cytoplasm. Recruited to focal adhesions upon activation.
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Research areas
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Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Cell Biology >> Apoptosis >> Intracellular >> Kinases
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Relevance
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The serine/threonine kinase Pak1 is an effector of small Rho GTPases, Rac1 and Cdc42. Pak1 complex specifically with Rac1 and Cdc42 in their active GTP bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. It plays an important role in the regulation of cell morphogenesis, motility, mitosis, and angiogenesis and has been implicated in the progression of many cancers.
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PAK1 (phospho T212) antibody (ab75599) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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ab75599 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against the non-phosphopeptide was removed by chromatography using a non-phosphopeptide corresponding to the phosphorylation site.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PAK1 (phospho T212) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PAK1 (phospho T212) antibody images, references, products related to ab75599 and other tools.
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PAK1 (phospho T212) antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAK1 (phospho T212) antibody (ab75599)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab75599, at 1/50 dilution, in the absence (left panel) and presence (right panel) of the immunising phosphopeptide.
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Western blot - PAK1 (phospho T212) antibody (ab75599)
All lanes : PAK1 (phospho T212) antibody (ab75599) at 1/500 dilution
Lane 1 : Extracts from 293 cells (untreated)
Lane 2 : Extracts from 293 cells treated with forskolin (40µM, 30min),
Predicted band size : 61 kDa
Observed band size : 65 kDa (why is the actual band size different from the predicted?)
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Immunocytochemistry/ Immunofluorescence - PAK1 (phospho T212) antibody (ab75599)
ab75599 at 1/100 dilution staining PAK1 (phospho T212) in human hela cells by ICC/IF.
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Search PubMed (MEDLINE) for references to PAK1
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PAK1 (phospho T212) antibody - more information
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Customer reviews (feedback) regarding PAK1 (phospho T212) antibody |
Customer FAQs regarding PAK1 (phospho T212) antibody |
Protocols for PAK1 (phospho T212) antibody |
Price and availability of products related to PAK1 (phospho T212) antibody |
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PAK1 (phospho T212) antibody - related products:
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PAK1 (phospho T212) antibody - other tools:
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Contact Abcam with a Technical Enquiry about PAK1 (phospho T212) antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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