![Western blot - PAK2 antibody [EP796Y] (ab76293) image](#ab76293_WB_1.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAK2 antibody [EP796Y] (ab76293) image](#ab76293_IHC_1.jpg)
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Products:Cell Biology >> Apoptosis >> Intracellular >> Kinases
Overview
Product name
Anti-PAK2 antibody [EP796Y]
See all PAK2 products (12) ...
Description
Rabbit monoclonal [EP796Y] to PAK2
Tested applications
WB, IHC-P, ICC, Flow Cytmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
A synthetic peptide corresponding to residues near the N terminus of human PAK2.
Positive control
WB: HeLa cytoplasmic lysate IHC-P; Human breast carcinoma tissue
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP796Y
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Cell Biology >> Apoptosis >> Intracellular >> Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab76293 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
Flow Cyt: 1/100.
ICC: 1/100 - 1/250.
IHC-P: 1/100 - 1/250. Antigen retrieval is recommended before commencing with IHC staining protocol. Use of HRP conjugated or polymerized HRP secondary antibody is recommended. Stronger signals have been found using the polymerized HRP secondary.
WB: 1/1000 - 1/2000. Predicted molecular weight: 58 kDa.
Is unsuitable for IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
The activated kinase acts on a variety of targets. Phosphorylates ribosomal protein S6, histone H4 and myelin basic protein. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. Caspase-activated PAK-2p34 is involved in cell death response, probably involving the JNK signaling pathway. Cleaved PAK-2p34 seems to have a higher activity than the CDC42-activated form.
Tissue specificity
Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen.
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain.
Post-translational
modifications
Full length PAK 2 is autophosphorylated when activated by CDC42/p21. Following cleavage, both peptides, PAK-2p27 and PAK-2p34, become highly autophosphorylated, with PAK-2p27 being phosphorylated on serine and PAK-2p34 on threonine residues, respectively. Autophosphorylation of PAK-2p27 can occur in the absence of any effectors and is dependent on phosphorylation of Thr-402, because PAK-2p27 is acting as an exogenous substrate.
During apoptosis proteolytically cleaved by caspase-3 or caspase-3-like proteases to yield active PAK-2p34.
Ubiquitinated, leading to its proteasomal degradation.
PAK-2p34 is myristoylated.
Cellular localization
Cytoplasm and Nucleus. Cytoplasm > perinuclear region. Membrane. Interaction with ARHGAP10 probably changes PAK-2p34 location to cytoplasmic perinuclear region. Myristoylation changes PAK-2p34 location to the membrane.
Target information above from: UniProt accessionQ13177
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- C-t-PAK2 antibody
- CB422 antibody
- EC 2.7.11.1 antibody
see all
Database links
- Entrez Gene: 5062 Human
- Entrez Gene: 224105 Mouse
- Entrez Gene: 29432 Rat
- Omim: 605022 Human
- SwissProt: Q13177 Human
- SwissProt: Q8CIN4 Mouse
- SwissProt: Q64303 Rat
- Unigene: 518530 Human
see all
Anti-PAK2 antibody [EP796Y] images:
Western blot - PAK2 antibody [EP796Y] (ab76293)
![Western blot - PAK2 antibody [EP796Y] (ab76293)](/ps/datasheet/Images/76/ab76293/ab76293_WB_1.jpg)
Anti-PAK2 antibody [EP796Y] (ab76293) at 1/2000 dilution + HeLa cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 58 kDa
Observed band size : 61 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAK2 antibody [EP796Y] (ab76293)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAK2 antibody [EP796Y] (ab76293)](/ps/datasheet/Images/76/ab76293/ab76293_IHC_1.jpg)
ab76293, at a 1/100 dilution, staining PAK2 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
References for Anti-PAK2 antibody [EP796Y] (ab76293)
ab76293 has not yet been referenced specifically in any publications.
Publishing research using ab76293? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"