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Anti-PAK2 antibody (ab19010)

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Overview

Product name

Anti-PAK2 antibody
See all PAK2 products (12) ...

Description

Rabbit polyclonal to PAK2

Specificity

This antibody recognises PAK2. It does not cross react with Pak1, Pak3, and Pak4 to 6.

Tested applications

WBmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide corresponding to the N terminus of human PAK2.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide.
Constituents: 50% Glycerol, PBS, BSA.

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Applications

Show applications key

Our Abpromise guarantee covers the use of ab19010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 1 - 2 µg/ml.Predicted molecular weight: 58 kDa.

Target

Function

The activated kinase acts on a variety of targets. Phosphorylates ribosomal protein S6, histone H4 and myelin basic protein. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. Caspase-activated PAK-2p34 is involved in cell death response, probably involving the JNK signaling pathway. Cleaved PAK-2p34 seems to have a higher activity than the CDC42-activated form.

Tissue specificity

Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen.

Sequence similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain.

Post-translational
modifications

Full length PAK 2 is autophosphorylated when activated by CDC42/p21. Following cleavage, both peptides, PAK-2p27 and PAK-2p34, become highly autophosphorylated, with PAK-2p27 being phosphorylated on serine and PAK-2p34 on threonine residues, respectively. Autophosphorylation of PAK-2p27 can occur in the absence of any effectors and is dependent on phosphorylation of Thr-402, because PAK-2p27 is acting as an exogenous substrate.
During apoptosis proteolytically cleaved by caspase-3 or caspase-3-like proteases to yield active PAK-2p34.
Ubiquitinated, leading to its proteasomal degradation.
PAK-2p34 is myristoylated.

Cellular localization

Cytoplasm and Nucleus. Cytoplasm > perinuclear region. Membrane. Interaction with ARHGAP10 probably changes PAK-2p34 location to cytoplasmic perinuclear region. Myristoylation changes PAK-2p34 location to the membrane.

Target information above from: UniProt accessionQ13177 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • C-t-PAK2 antibody
  • CB422 antibody
  • EC 2.7.11.1 antibody
  • Gamma PAK antibody
  • Gamma-PAK antibody
  • hPAK65 antibody
  • Kinase antibody
  • p21 (CDKN1A)-activated kinase 2a antibody
  • p21 activated kinase 2 antibody
  • p21 protein (Cdc42/Rac)-activated kinase 2 antibody
  • p21 protein Cdc42 Rac activated kinase 2 antibody
  • p21-activated kinase 2 antibody
  • p21-activated kinase, 65-KD antibody
  • p21-activated protein kinase I antibody
  • p21CDKN1A activated kinase 2 antibody
  • p27 antibody
  • p34 antibody
  • p58 antibody
  • p65PAK antibody
  • PAK 2 antibody
  • PAK-2 antibody
  • PAK-2p34 antibody
  • Pak2 antibody
  • PAK2_HUMAN antibody
  • PAK65 antibody
  • PAKgamma antibody
  • S6 H4 kinase antibody
  • S6/H4 kinase antibody
  • Serine threonine protein kinase PAK 2 antibody
see all

References for Anti-PAK2 antibody (ab19010)

ab19010 has not yet been referenced specifically in any publications.

Publishing research using ab19010? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"