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Western blot - PAK2 antibody (ab52811)
Western blot - PAK2 antibody (ab52811)
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Product Name 

PAK2 antibody

 

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Product type 

Primary antibodies

Description 

Mouse polyclonal to PAK2

Immunogen 

Vector coding for a partial recombinant fusion protein corresponding to amino acids 47-65|143-156|205-221 from Human PAK2.
Target sequence used to make the antibody: KKPRHKIISI FSGTEKGSKT PPEKDGLPSG TPAPVGDSHV DGAAKSLDKQ.
BLAST 'KKPRHKIISI FSGTEKGSKT PPEKDGLPSG TPAPVGDSHV DGAAKSLDKQ ' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 

Hu

Predicted to react 
(species key)

Ms
(Due to sequence homology)

Tested applications 
(see key)

WB


Abreviews 

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Application notes 
(see key)

Recommended dilutions
WB: 1/1000. Predicted molecular weight: 58 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Cell Biology >> Apoptosis >> Intracellular >> Kinases

Relevance 

The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase mediated apoptosis, PAK2 also interacts with and is activated by HIV1 Nef.

This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein.
A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein.
Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

 

Alternative names PAK2 antibody (ab52811)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

5062    (Human)
224105    (Mouse)

Omim

    

605022    (Human)

SwissProt

    

Q13177    (Human)
Q8CIN4    (Mouse)

Unigene

    

518530    (Human)

Raised in 

Mouse

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Preservative: None
Constituents: 50% Glycerol, Whole serum

Material safety datasheet (MSDS) for this product:
Glycerol MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PAK2 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for PAK2 antibody images, references, products related to ab52811 and other tools.

 

PAK2 antibody images:

Product image  Western blot - PAK2 antibody (ab52811)

Western blot - PAK2 antibody (ab52811)

All lanes : PAK2 antibody (ab52811) at 1/1000 dilution

Lane 1 : ~20ug of a total protein extract from E coli with ~50ng to 100ng of a tagged fusion protein of an irrelevant antigen.
Lane 2 : ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (tagged-PAK2 fusion protein).

Secondary
HRP conjugated Rabbit anti-mouse IgG + IgM (H+L) at 1/5000 dilution

Predicted band size : 58 kDa
Observed band size : 32.5 kDa (why is the actual band size different from the predicted?)


The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to an enzyme tag.

 
 

Search PubMed (MEDLINE) for references to PAK2

 

PAK2 antibody - more information

Customer reviews (feedback) regarding PAK2 antibody

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Price and availability of products related to PAK2 antibody

 

PAK2 antibody - related products:

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