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All lanes : Anti-PAK2 (phospho S20) antibody (ab59359) at 1/500 dilution
Lane 1 : Extracts from 293 cells, treated
with Sorbitol (0.4M, 30min) with no immunizing peptide
Lane 2 : Extracts from 293 cells, treated
with Sorbitol (0.4M, 30mins) with immunizing peptide
Observed band size : 58 kDa (why is the actual band size different from the predicted?)
ab59359, at a 1/50 dilution, staining human PAK2 (phospho S20) in colon carcinoma, using Immunohistochemistry, Paraffin embedded tissue, in the absence of the immunizing peptide (left image) and in the presence of the immunizing peptide (right image).
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