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Products:Cell Biology >> Apoptosis >> Intracellular >> Kinases
Overview
Product name
Anti-PAK2 (phospho S20) antibody
See all PAK2 products (12) ...
Description
Rabbit polyclonal to PAK2 (phospho S20)
Specificity
ab59359 detects endogenous levels of PAK2 only when phosphorylated at serine 20.
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthesized phosphopeptide derived from human PAK2 around the phosphorylation site of serine 20 (MSSPTI).
Positive control
293 whole cell lysate (WB) Human colon carcinoma tissue (IHC)
General notes
Store at -20°C for 1 year
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg+2 and Ca+2), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Cell Biology >> Apoptosis >> Intracellular >> Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab59359 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 58 kDa.
ELISA
ELISA: 1/40000ELISA: 1/40000
IHC-P: 1/50 - 1/100.
Target
Function
The activated kinase acts on a variety of targets. Phosphorylates ribosomal protein S6, histone H4 and myelin basic protein. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. Caspase-activated PAK-2p34 is involved in cell death response, probably involving the JNK signaling pathway. Cleaved PAK-2p34 seems to have a higher activity than the CDC42-activated form.
Tissue specificity
Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen.
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain.
Post-translational
modifications
Full length PAK 2 is autophosphorylated when activated by CDC42/p21. Following cleavage, both peptides, PAK-2p27 and PAK-2p34, become highly autophosphorylated, with PAK-2p27 being phosphorylated on serine and PAK-2p34 on threonine residues, respectively. Autophosphorylation of PAK-2p27 can occur in the absence of any effectors and is dependent on phosphorylation of Thr-402, because PAK-2p27 is acting as an exogenous substrate.
During apoptosis proteolytically cleaved by caspase-3 or caspase-3-like proteases to yield active PAK-2p34.
Ubiquitinated, leading to its proteasomal degradation.
PAK-2p34 is myristoylated.
Cellular localization
Cytoplasm and Nucleus. Cytoplasm > perinuclear region. Membrane. Interaction with ARHGAP10 probably changes PAK-2p34 location to cytoplasmic perinuclear region. Myristoylation changes PAK-2p34 location to the membrane.
Target information above from: UniProt accessionQ13177
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- C-t-PAK2 antibody
- CB422 antibody
- EC 2.7.11.1 antibody
see all
Database links
- Entrez Gene: 5062 Human
- Entrez Gene: 224105 Mouse
- Entrez Gene: 29432 Rat
- Omim: 605022 Human
- SwissProt: Q13177 Human
- SwissProt: Q8CIN4 Mouse
- SwissProt: Q64303 Rat
- Unigene: 518530 Human
see all
Anti-PAK2 (phospho S20) antibody images:
Western blot - PAK2 (phospho S20) antibody (ab59359)
All lanes : Anti-PAK2 (phospho S20) antibody (ab59359) at 1/500 dilution
Lane 1 : Extracts from 293 cells, treated
with Sorbitol (0.4M, 30min) with no immunizing peptide
Lane 2 : Extracts from 293 cells, treated
with Sorbitol (0.4M, 30mins) with immunizing peptide
Observed band size : 58 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - PAK2 (phospho S20) antibody (ab59359)
ab59359, at a 1/50 dilution, staining human PAK2 (phospho S20) in colon carcinoma, using Immunohistochemistry, Paraffin embedded tissue, in the absence of the immunizing peptide (left image) and in the presence of the immunizing peptide (right image).
References for Anti-PAK2 (phospho S20) antibody (ab59359)
ab59359 has not yet been referenced specifically in any publications.
Publishing research using ab59359? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"