![Western blot - PAK3 antibody [EP797Y] (ab40808) image](#ab40808_1.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAK3 antibody [EP797Y] (ab40808) image](#ab40808_2.jpg)
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Products:Neuroscience >> Neurology process >> Neural Signal Transduction
Overview
Product name
Anti-PAK3 antibody [EP797Y]
See all PAK3 products (6) ...
Description
Rabbit monoclonal [EP797Y] to PAK3
Tested applications
WB, IHC-P, ICC, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide corresponding to residues in the N terminus of Human PAK3.
Positive control
Fetal brain lysate and human brain glioma.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP797Y
Isotype
IgG
Research areas
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Neuroscience >> Neurology process >> Neural Signal Transduction
Applications
Show applications key
Our Abpromise guarantee covers the use of ab40808 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC: 1/100 - 1/250.
IHC-P: 1/100 - 1/250.
IP: 1/50.
WB: 1/10000 - 1/50000. Detects a band of approximately 65 kDa (predicted molecular weight: 62 kDa).
Is unsuitable for FACS.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities. PAK3 forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1 proteins which then catalyzes a variety of targets. It may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. A point mutation in this gene has been linked to nonsyndromic X-linked mental retardation.
Cellular localization
Cytoplasmic
Alternative names
- Beta PAK antibody
- bPAK antibody
- CDKN1A antibody
see all
Database links
- Entrez Gene: 5063 Human
- Entrez Gene: 18481 Mouse
- Entrez Gene: 29433 Rat
- Omim: 300142 Human
- SwissProt: O75914 Human
- SwissProt: Q17RE5 Human
- SwissProt: Q61036 Mouse
- SwissProt: Q62829 Rat
see all
Anti-PAK3 antibody [EP797Y] images:
Western blot - PAK3 antibody [EP797Y] (ab40808)
![Western blot - PAK3 antibody [EP797Y] (ab40808)](/ps/datasheet/Images/40/ab40808/ab40808_1.jpg)
Anti-PAK3 antibody [EP797Y] (ab40808) at 1/50000 dilution + fetal brain lysate at 10 µg
Predicted band size : 62 kDa
Observed band size : 65 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAK3 antibody [EP797Y] (ab40808)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAK3 antibody [EP797Y] (ab40808)](/ps/datasheet/Images/40/ab40808/ab40808_2.jpg)
ab40808 at a 1:100 dilution staining PAK3 in human brain glioma.
References for Anti-PAK3 antibody [EP797Y] (ab40808)
ab40808 has not yet been referenced specifically in any publications.
Publishing research using ab40808? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"